Which assumptions need to be fulfilled while Lavaan in SEM/Path Analysis ?

Hello Everyone, What are the assumptions I need to fulfill for SEM analysis or path analysis using Lavaan in R?

03 February 2021 4,001 2 View

How to report output from Kruskal Wallis pairwise comparisons from SPSS?

Hi, I am currently in the process of writing up my results from a research project. The assumptions for running a Manova were violated so I used a series of Kruskal Wallis tests using SPSS. I...

22 March 2020 5,242 3 View

How can we design a competency based medical education for the basic medical sciences courses (anatomy, physiology, microbiology etc)?

Most of the existing literature explains the design and implementation of CBME in clinical phases or the residency programs. How to design CBME for Basic Medical Sciences Courses? when writing the...

25 June 2019 5,316 21 View

Hello everyone, please let me know how can i obtain pure protein in case of high sugar sample???

(my sample is protein bar formulated with dates apricots cheese and whey protein Isolate. I am interested to study the protein and peptides in my product through electrophrosis. So, please let me...

08 November 2018 6,841 4 View

Is anyone study on HepG2 and renal cell line toxicity by inducing chromium?

I'm concentrating on HepG2 and renal cell line toxicity by inducing a chemical., for i need papers pertaining to my study.If anybody possessing articles on these please provide me the same.

20 January 2018 673 1 View

How to purify bacterial RNA (mRNA) from the infected blood of buffaloes, free of Eukaryotic RNA to run NGS for RNA sequencing.

I am struggling to purify bacterial RNA (mRNA) from the infected blood of buffaloes.My concerned is that my bacterial RNA must be free of Eukaryotic RNA from blood and need more RNA concentration...

06 March 2016 5,539 3 View

Are there any techniques, other than ELISA, for measuring secreted protein (21KDa) from the conditioned media?

Since ELISA kits are very expensive, was wondering if I could measure that with western blot technique? and If yes, how to measure the protein concentration? For the cell lysate I use protease...

06 April 2015 2,460 7 View

BrdU or Nestin: which one is better to use as marker for proliferation assay?

Since both markers can be used to check the proliferation of neural stem cells, I was wondering what is the difference in using them? Is there a difference in the method using it or price? Can...

23 September 2013 5,826 7 View

Using median of controls as ref value for single sample t-test.

I need your advice regarding the appropriateness of following statistical procedures: One of our studies aimed to assess indicators of metabolic syndrome at birth in two groups of neonates. As...

18 April 2013 7,518 2 View

Can we estimate the statistical distribution of growth associated and non growth associated constants for bacterial growth?

Reviewer of my research article asked about the distribution (either normal or not normal) of some values including the growth associated and non growth associated constants for bacterial growth....

10 March 2013 8,927 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View