15 Questions 17 Answers 0 Followers
Questions related from Ping Kwan
Dear colleagues, I was wondering if anyone can recommend to me a literature or directly tell me, what is the actual reflection/meaning of the following electrophysiological features in a MEPP peak...
08 February 2016 1,502 2 View
What is the intracellular distribution of Matrix Metalloproteinase (MMP)-9 in fast motor neurons (if possible, which organelle)? Do they found in the axon terminal? Additionally, do microglia...
29 September 2015 7,404 3 View
I am recently planning to do some stereotaxic injections to some mice brain nuclei. Due to the various choices (in particular the G number) of Hamilton needles, I was wondering which one would be...
01 March 2015 6,008 3 View
In some in vivo patch clamp paper, they often describes a range of resting potential in which the cells are considered healthy and can be used for further analysis. I was wondering how such...
01 February 2015 3,732 3 View
Greetings. I was wondering whether there is a table containing a list of extracellular ions and intracellular ions? More importantly, if my questions are: how many different ions present in the...
10 January 2015 4,913 1 View
Recently I am doing some in vivo patch clamping. Unlike in vitro one, in vivo patch clamping is virtually blind except the information regarding the resistance. Despite I have a very high success...
16 December 2014 1,848 8 View
To the best of my knowledge, biocytin can be used to identify the patched cell but only after the histology staining. As I would like to label/mark the living cell that have been patched, I was...
14 December 2014 2,574 4 View
I am currently working with flowering plants, as I need to quantify the content of chlorophyll (particular subtype, e.g. chlorophyll a) in a specific leaf after its extraction, I was wondering...
13 December 2014 2,356 12 View
I have a drug that is non-polar in nature (dissolved in DMSO or acetone), but I would like it to be incorporated in my internal solution and my bath (which is polar in nature). I was wondering...
13 December 2014 7,452 4 View
As seen in some labs, people do hippocampal slices for their patch clamping on hippocampal neurons. I was wondering what the significance is of making the hippocampal slices instead of using a...
09 December 2014 8,331 5 View
By convention, 5-8Mohm of 1-2 um glass microelectrode shall be used for patching the pyramidal neurons in the brain (please correct me if I were wrong). However, due to some adjustments to the...
30 November 2014 3,643 13 View
In patch clamps, operators are required to make a very-fine-tipped glass micropipette for their electrode. Due to the small bore of the glass tip (around 1-2 um), air bubbles are sometimes trapped...
30 November 2014 4,798 9 View
After the first (agarose or SDS-PAG) electrophoresis, if I run the same gel again with an-overtime so that everything in the gel will now in theory come off the gel (i.e. electro-rinsing), then...
06 March 2014 992 10 View
If watercrystal damage happened in my whole brain samples, would there be any significant differences in the result of my immunoblotting between step A and step B (which are as follows)? Step A:...
12 February 2014 8,524 2 View
(i.e. Without any artificial interventions)
19 March 2013 6,201 4 View
