Ping Kwan

11 Questions 17 Answers 0 Followers

Questions related from Ping Kwan

What is the intracellular distribution of Matrix Metalloproteinase (MMP)-9 in fast motor neurons?

What is the intracellular distribution of Matrix Metalloproteinase (MMP)-9 in fast motor neurons (if possible, which organelle)? Do they found in the axon terminal? Additionally, do microglia...

29 September 2015 7,330 3 View

What is the prefered/best guage of Hamilton needle used for mice brain injections?

I am recently planning to do some stereotaxic injections to some mice brain nuclei. Due to the various choices (in particular the G number) of Hamilton needles, I was wondering which one would be...

01 March 2015 5,945 3 View

Is there a technique for staining living cell for patching clamping?

To the best of my knowledge, biocytin can be used to identify the patched cell but only after the histology staining. As I would like to label/mark the living cell that have been patched, I was...

14 December 2014 2,522 4 View

What is the extraction protocol for chlorophyll with high purity?

I am currently working with flowering plants, as I need to quantify the content of chlorophyll (particular subtype, e.g. chlorophyll a) in a specific leaf after its extraction, I was wondering...

13 December 2014 2,299 12 View

How to add a non-polar substance in my patch clamp internal solution?

I have a drug that is non-polar in nature (dissolved in DMSO or acetone), but I would like it to be incorporated in my internal solution and my bath (which is polar in nature). I was wondering...

13 December 2014 7,390 4 View

Should you patch hippocampal neurons in acute brain slices or hippocampal slices?

As seen in some labs, people do hippocampal slices for their patch clamping on hippocampal neurons. I was wondering what the significance is of making the hippocampal slices instead of using a...

09 December 2014 8,285 5 View

Is the 8-9Mohm glass microelectrode suitable for patch clamping?

By convention, 5-8Mohm of 1-2 um glass microelectrode shall be used for patching the pyramidal neurons in the brain (please correct me if I were wrong). However, due to some adjustments to the...

30 November 2014 3,598 13 View

Any tips/techniques to prevent the air bubble trap in the glass micropipette?

In patch clamps, operators are required to make a very-fine-tipped glass micropipette for their electrode. Due to the small bore of the glass tip (around 1-2 um), air bubbles are sometimes trapped...

30 November 2014 4,745 9 View

Can anyone help with electrophoresis gel reuse?

After the first (agarose or SDS-PAG) electrophoresis, if I run the same gel again with an-overtime so that everything in the gel will now in theory come off the gel (i.e. electro-rinsing), then...

06 March 2014 938 10 View

Anyone have experience with immunoblot for watercrystal damaged samples?

If watercrystal damage happened in my whole brain samples, would there be any significant differences in the result of my immunoblotting between step A and step B (which are as follows)? Step A:...

12 February 2014 8,457 2 View

Can the sympathetic / parasympathetic nervous system be partially / locally activated in the case of natural conditions?

(i.e. Without any artificial interventions)

19 March 2013 6,161 4 View