Rubina Shakya

8 Questions 14 Answers 0 Followers

Questions related from Rubina Shakya

How to make sure the frequency of the binaural beats of 40 Hz in the videos uploaded on youtube?

I am planning to do an experiment on gamma wave binaural beats of 40 Hz. I have downloaded this video https://www.youtube.com/watch?v=mQXNCwfqRjo from youtube. I was wondering if anybody could...

24 September 2018 6,318 4 View

What are the average concentration of hormones especially estradiol in fetal bovine serum?

We have been using 10% FBS (Gibco) + MEM/F12 media for the study related with effect of estradiol in SH-SY5Y neuroblastoma cell proliferation. Now, the charcoal stripped FBS concept is confusing...

15 September 2018 9,249 0 View

After protein precipitation why the pellet can not easily be dissolved with sample buffer (loading dye) for western blot?

I tried protein precipitation with TCA (250ul in 900 ul of sample volume) and washed with Acetone 4 times..the pellet is not completely dissolved in the sample buffer. 1. I put it on the hot plate...

23 May 2015 6,577 14 View

Are there any techniques, other than ELISA, for measuring secreted protein (21KDa) from the conditioned media?

Since ELISA kits are very expensive, was wondering if I could measure that with western blot technique? and If yes, how to measure the protein concentration? For the cell lysate I use protease...

07 April 2015 2,564 7 View

How the water soluble estradiol is different from the alcohol soluble estradiol?

I have been using water soluble estradiol for the cell proliferation assay in 5Y cell line. My questions are; 1. Can we store the stock solution of estradiol in plastic container (microfuge...

22 November 2014 6,604 2 View

BrdU or Nestin: which one is better to use as marker for proliferation assay?

Since both markers can be used to check the proliferation of neural stem cells, I was wondering what is the difference in using them? Is there a difference in the method using it or price? Can...

24 September 2013 5,906 7 View

How to coculture hippocampal and microglial cell lines?

Since hippocampal and microglial two cell lines are maintained in different temperature, I was wondering how to conduct the experiment having coculture between these two.

22 September 2013 9,285 0 View

Which rodent hippocampal cell line is suitable for working on the effect of estrogen?

I came to know that certain hippocampal cell lines don't express estrogen receptors (such as HT22). I was thinking of working on rodent cell line SCR022, but no information about the presence of...

03 September 2013 2,208 7 View