27 October 2022 1 5K Report

We are looking for MinION sequencing but we don't have experience for which QIAGEN Kit to use. It's for Acinetobacter baumannii

More Dafne Abigaíl Guillén Navarro's questions See All
Personal recommendations for GAPDH antibody?

Hello! I am planning a western blot experiment, and I am looking for GAPDH antibodies to order. In the past, I have experienced a lot of hit-or-miss when ordering antibodies based on just the...

07 July 2024 1,685 0 View

PVDF or nitrocellulose to visualize phosphorylated proteins?

Which membrane material is the best for capturing and visualizing phosphorylated proteins in a western blot? Or is there no difference?

05 June 2024 9,502 2 View

Best primers for human genes COXI and COXIV?

Hello everyone! My PI would like me to measure the amount of mitochondrial DNA in multiple cell lines using qPCR targeting COXI and COXIV. However, I have never done a qPCR that measures...

03 June 2024 6,795 1 View

Best apps for RNAseq heatmap generation in Illumina BaseSpace?

Hello! I am trying to analyze some RNAseq data I recently received. I would like to craft a heatmap comparing the expression of specific genes across my cell samples, but I am not sure which of...

08 May 2024 5,665 0 View

What is the best way to normalize p-S6(Ser235/236) protein expression?

Hello everyone. I am in the middle of performing western blotting experiments measuring expression of p-S6(Ser235/236) protein in total cell lysate. Originally, I was quantifying my data using...

11 April 2024 4,457 1 View

15 mL conical tubes & Eppendorf tubes compatible with liquid nitrogen and dry ice?

Hello! I need to snap freeze cancer pellets to submit for multiomics. Does anyone know of 15 mL conical tubes or Eppendorf/microcentrifuge tubes that can withstand liquid nitrogen and dry ice?

15 February 2024 4,199 1 View

How do I know if my sample is reduced or non-reduced for western blot?

Hello! I am not sure whether or not my protein samples are reduced or not. Based on the information below, can someone please let me know if they are reduced? Sample setup: __ uL total cell...

12 February 2024 4,912 1 View

I can't make this solution. I need help with the concentrations?

Hello all! I need to make 100 mL of the following solution. I tried to use the C1V1 formula, but I have dyscalculia so my math skills are non-existent. Ponceau-stained membrane can be detained...

01 February 2024 8,962 1 View

Can convergence (SCF) be improved for transitional metals?

In Guassian, which convergence algorithm is best for working with transition metal interaction? It is taking a long time to even get the first block of coordinates, in the output file it said to...

28 January 2024 4,276 1 View

Recommendations for protein markers of cell stress/starvation?

Hello everyone! I'm currently running an in vitro experiment where I test the effects of treating human cancer cells with "starvation media" (aka DMEM media with no FBS) on mTORC1 signaling. I...

18 January 2024 4,365 1 View

Similar questions and discussions
Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

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