Why am I not able to separate His6-MBP from the protein of my interest?

My protein is of ~11 kDa. While eluting from the Gel filtration column, It comes with the MBP tag. I did cleavage to separate tag from the protein. So other impurities are removed but the MBP tag...

07 August 2015 978 13 View

How can I calculate the thermodynamic parameters of double stranded RNA?

I want to compare the thermodynamics of the two end of the dsRNA. Is there any software available? Is there any reference paper by which I can find the local thermodynamic parameters of the RNA at...

01 February 2015 5,571 3 View

Can any one explain how to get the dwell time plots from the FRET trajectories?

How do I convert the fret Eapp data to the dwell time?

10 November 2014 4,827 2 View

Can I use EDTA and/or DTT in wash buffer and elution buffer used for Ni affinity chromatography?

I need to purify His6 tag containing protein. Normally I use lysis buffer containing EDTA and DTT for preparing cell lysate. So can I use same buffer by adding imidazole for chromatographic...

08 September 2014 6,006 8 View

How can I purify MBP-TEV tagged protein expressed from BL21 cells?

Is there any other way than affinity chromatography to separate the protein? How much TEV to protein ratio can be used for TEV cleavage? Also can any one suggest the conditions to be used for the...

08 September 2014 7,922 7 View

Does anyone know any HPLC methods having a mobile phase with the combination methanol/water or acn/water?

I want to study these methods for a project. Please also give retention time and retention factor for as many drugs as available.

06 July 2013 568 3 View

is there any reference where I can get UV spectra for the various molecule?

I am interested for the UV spectra of vitamins.

02 March 2013 9,658 3 View

Can anyone tell me how to determine the LOQ and LOD for a HPLC assay method, practically?

Is there any way other than prediction from signal to noise to ratio? In case of s/n, the s/n for the peak near its base should be considered or the ratio should be calculated from the base line...

01 February 2013 5,957 17 View

How can we distinguish between different types of columns?

for example, ODS, synchronus, hypersil, nucleosil, etc.

31 December 2012 641 10 View

How do I develop an in house standard for a product? How do I interpret the available data?

I have collected the physical and chemical data from three batches. Is this sufficient for the development of in house standards? how do I proceed with the data available?

11 December 2012 2,436 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View