is there any reference where I can get UV spectra for the various molecule?

More Harshad Paithankar's questions See All

Why am I not able to separate His6-MBP from the protein of my interest?

My protein is of ~11 kDa. While eluting from the Gel filtration column, It comes with the MBP tag. I did cleavage to separate tag from the protein. So other impurities are removed but the MBP tag...

07 August 2015 1,027 13 View

How can I calculate the thermodynamic parameters of double stranded RNA?

I want to compare the thermodynamics of the two end of the dsRNA. Is there any software available? Is there any reference paper by which I can find the local thermodynamic parameters of the RNA at...

01 February 2015 5,631 3 View

Can any one explain how to get the dwell time plots from the FRET trajectories?

How do I convert the fret Eapp data to the dwell time?

10 November 2014 4,875 2 View

Can I use EDTA and/or DTT in wash buffer and elution buffer used for Ni affinity chromatography?

I need to purify His6 tag containing protein. Normally I use lysis buffer containing EDTA and DTT for preparing cell lysate. So can I use same buffer by adding imidazole for chromatographic...

08 September 2014 6,054 8 View

How can I purify MBP-TEV tagged protein expressed from BL21 cells?

Is there any other way than affinity chromatography to separate the protein? How much TEV to protein ratio can be used for TEV cleavage? Also can any one suggest the conditions to be used for the...

08 September 2014 7,966 7 View

Which proteins can be separated by using reverse phase HPLC?

Is there any comparative study of protein purification or separation by using reverse phase HPLC and fast protein liquid chromatography (FPLC)? What are the criteria for separation of protein by...

02 March 2014 2,317 1 View

Does anyone know any HPLC methods having a mobile phase with the combination methanol/water or acn/water?

I want to study these methods for a project. Please also give retention time and retention factor for as many drugs as available.

06 July 2013 620 3 View

Can anyone tell me how to determine the LOQ and LOD for a HPLC assay method, practically?

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How can we distinguish between different types of columns?

for example, ODS, synchronus, hypersil, nucleosil, etc.

31 December 2012 702 10 View

How do I develop an in house standard for a product? How do I interpret the available data?

I have collected the physical and chemical data from three batches. Is this sufficient for the development of in house standards? how do I proceed with the data available?

11 December 2012 2,486 0 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

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11 August 2024 9,801 2 View

Baseline drift in HPLC? What causes this?

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11 August 2024 3,770 4 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

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05 August 2024 3,783 2 View

Can a Conventional 50W visible LED Lamp Emit UV Light?

I'm researching light sources and their UV emissions. I'm curious if a 50W, 7500K conventional LED lamp emits any UV light. I found that a 200W tungsten lamp can emit up to 1.5 mW/cm² at 350 nm....

31 July 2024 3,843 7 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

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31 July 2024 9,140 2 View

How to calculate conductivity using Electrochemical Impedance Spectroscopy's Nyquist plot?

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29 July 2024 1,376 0 View

How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program?

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28 July 2024 9,165 2 View

Why semicircle of nyquist plot is not complete?

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What is single absorption ESR spectroscopy ?

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25 July 2024 2,325 1 View

Can we fit a gaussian Fit on a LIBS Peak ?

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23 July 2024 6,127 1 View

Teodoro S Kaufman

Try sbds

Serdar Badoğlu

SDBS is a nice source, check it out at

http://sdbs.riodb.aist.go.jp/sdbs/cgi-bin/direct_frame_top.cgi

Harry ten Brink

Theodore / Serdar

I did not find UV-spectra in SDBS?!

Furthermore it provides an IR-spectrum of my favorite molecule, glyoxal, as a water solution; but then glyoxal is present in the trimere-form, not the pure monomere