03 March 2013 3 10K Report

I am interested for the UV spectra of vitamins.

More Harshad Paithankar's questions See All
Why am I not able to separate His6-MBP from the protein of my interest?

My protein is of ~11 kDa. While eluting from the Gel filtration column, It comes with the MBP tag. I did cleavage to separate tag from the protein. So other impurities are removed but the MBP tag...

07 August 2015 978 13 View

How can I calculate the thermodynamic parameters of double stranded RNA?

I want to compare the thermodynamics of the two end of the dsRNA. Is there any software available? Is there any reference paper by which I can find the local thermodynamic parameters of the RNA at...

01 February 2015 5,571 3 View

Can any one explain how to get the dwell time plots from the FRET trajectories?

How do I convert the fret Eapp data to the dwell time?

10 November 2014 4,827 2 View

Can I use EDTA and/or DTT in wash buffer and elution buffer used for Ni affinity chromatography?

I need to purify His6 tag containing protein. Normally I use lysis buffer containing EDTA and DTT for preparing cell lysate. So can I use same buffer by adding imidazole for chromatographic...

08 September 2014 6,006 8 View

How can I purify MBP-TEV tagged protein expressed from BL21 cells?

Is there any other way than affinity chromatography to separate the protein? How much TEV to protein ratio can be used for TEV cleavage? Also can any one suggest the conditions to be used for the...

08 September 2014 7,922 7 View

Which proteins can be separated by using reverse phase HPLC?

Is there any comparative study of protein purification or separation by using reverse phase HPLC and fast protein liquid chromatography (FPLC)? What are the criteria for separation of protein by...

02 March 2014 2,263 1 View

Does anyone know any HPLC methods having a mobile phase with the combination methanol/water or acn/water?

I want to study these methods for a project. Please also give retention time and retention factor for as many drugs as available.

06 July 2013 568 3 View

Can anyone tell me how to determine the LOQ and LOD for a HPLC assay method, practically?

Is there any way other than prediction from signal to noise to ratio? In case of s/n, the s/n for the peak near its base should be considered or the ratio should be calculated from the base line...

01 February 2013 5,957 17 View

How can we distinguish between different types of columns?

for example, ODS, synchronus, hypersil, nucleosil, etc.

31 December 2012 641 10 View

How do I develop an in house standard for a product? How do I interpret the available data?

I have collected the physical and chemical data from three batches. Is this sufficient for the development of in house standards? how do I proceed with the data available?

11 December 2012 2,436 0 View

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