Which platform is better for performing microarray data analysis? agilent, affymetrix or illumina?

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Which is true nano nitrogen or nano urea?

Some scientists use nano nitrogen and some use nano urea, even though the process used the same for preparation. So, which is true nano nitrogen or nano urea?

28 June 2024 2,885 6 View

What analyses should I perform (in spss) on my pretest data to select products for my main study?

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06 June 2023 7,355 4 View

How to identify the batch informations from the sample ID of TCGA database?

I have thousands of samples from `TCGA` retrieved using `TCGABiolinks`. I want to remove the `batch effect` from the datasets. It's mentioned that batch can be detected from sample ID itself How...

08 March 2023 3,496 0 View

How to classify Tumor Mutation Burden into TMB high, medium and low groups ?

Tumor mutational burden (TMB) is a measure of the number of gene mutations inside the cancer cells. TMB of a tumor sample is calculated by the number of non-synonymous somatic mutations per...

01 September 2020 4,826 1 View

How to extract list of proteins delectable by immune histochemistry?

Is there any database or tool where I can retrieve list of proteins detectable by immune histochemistry (IHC)?

02 August 2020 7,840 2 View

How to remove a non-ionic surfactant (eg-tween20) from the reaction mixture?

I attempted to prepare CuS nanomaterials using microemulsion method. I used tween20 as the surfactant. But found it is very difficult to remove the surfactant from microemulsion. How can we remove...

23 June 2020 5,758 17 View

How to measure Lowest Detection Limit of a sensor material in Electrochemical sensing?

I know Differential Pulse Voltametry is using to measure LOD value. But I do not know how to measure the Lowest Detection Limit of a sensor in Electrochemical sensing. Please reply. Thanks.

09 March 2020 3,676 3 View

What is the longest deletion or insertion that can be detected in next generation sequencing data analysis?

what is the longest deletion or insertion that can be detected in next generation sequencing data analysis

26 February 2020 3,113 3 View

What is the possibility of replicates being different from other replicates of same sample?

I have analyzed the dataset of GSE38132 from gene expression omnibus. The data is from cell line breast cancer ZR-75-1 which comprises of 9 conditions with 4 replicates for each condition making...

16 February 2020 5,954 4 View

How to perform normalization and calculate when there are multiple groups ?

I have 4 sample [control, pre-treatment, post-treatment, metastais] with 4 replicates which make total 4x4 = 16 samples I want to perform comparison of control with all other groups. For the said...

12 February 2020 6,528 2 View

Analysis of MHC-I and II alleles with CNVs and unassigned loci?

I am working on a dataset of MHC-I and II alleles from a bird species sequenced with Illumina. We were not able to assign alleles to loci through MHC-typer as we were over the limit of 150 alleles...

15 July 2024 182 1 View

Who can recommend where to buy Flow Cells for Illumina HiSeq 2500?

I recently acquired a used Illumina HiSeq 2500 and am in need of flow cells compatible with this machine. As I am relatively new to this aspect, I would greatly appreciate any recommendations on...

22 June 2024 5,992 1 View

How can I purify filamentous fungi from leaf tissue?

I want to perform PacBio RNA-seq of a filamentous fungal isolate during multiple infection time-points in barley leaf. I was told that PacBio RNA-seq would not be viable due to the low...

12 June 2024 4,027 1 View

What is the Ideal cover in a shotgun soil metagenomic?

Hello everyone, I am starting a soil mapping project in a specific crop region, and my goal is to perform shotgun sequencing (Illumina NovaSeq) to identify microorganisms and potential genes. This...

24 May 2024 5,021 4 View

Best apps for RNAseq heatmap generation in Illumina BaseSpace?

Hello! I am trying to analyze some RNAseq data I recently received. I would like to craft a heatmap comparing the expression of specific genes across my cell samples, but I am not sure which of...

08 May 2024 5,665 0 View

How to get the annotation file of the Immunochip array?

Dear community, I'm inputting data from an old GWAS study based on the Immunochip array, and I need the annotation file to conduct a few QC steps. I can't find it on the Illumina website; I...

06 April 2024 8,979 2 View

Whats wrong with the Illumina sequencing?

I recently performed Illumina shotgun sequencing and my fastqc data is as attached. is this correct?

01 April 2024 3,354 6 View

PhD candidate with a research focus on chronic inflammation particularly macrophage cells so i need professor information this area??

I am a PhD candidate in immunology at AWKUM with a research focus on chronic inflammation, particularly the role of macrophage cells. I am writing to express my keen interest in your research on...

28 March 2024 7,798 0 View

Finding the differentially expressed genes?

1. For the gene expression data (microarray dataset which is been extracted from the Gene Expression Omnibus (GEO) platform), which of the following normalisation techniques are suggested as the...

05 March 2024 8,451 1 View

What types of graphs are commonly used when wanting to visualize data on lectin microarrays and glycans?

I would like to know what types of graphs are commonly used when wanting to visualize data on lectin microarrays and glycans. The goal is to compare samples from patients with lung cancer and...

21 February 2024 7,127 1 View