How to extract list of proteins delectable by immune histochemistry?

More Snijesh V.P.'s questions See All

Which is true nano nitrogen or nano urea?

Some scientists use nano nitrogen and some use nano urea, even though the process used the same for preparation. So, which is true nano nitrogen or nano urea?

28 June 2024 2,885 6 View

What analyses should I perform (in spss) on my pretest data to select products for my main study?

...

06 June 2023 7,355 4 View

How to identify the batch informations from the sample ID of TCGA database?

I have thousands of samples from `TCGA` retrieved using `TCGABiolinks`. I want to remove the `batch effect` from the datasets. It's mentioned that batch can be detected from sample ID itself How...

08 March 2023 3,496 0 View

Which platform is better for performing microarray data analysis? agilent, affymetrix or illumina?

which platform is better for performing microarray data analysis? agilent, affymetrix or illumina

09 April 2021 3,894 3 View

How to classify Tumor Mutation Burden into TMB high, medium and low groups ?

Tumor mutational burden (TMB) is a measure of the number of gene mutations inside the cancer cells. TMB of a tumor sample is calculated by the number of non-synonymous somatic mutations per...

01 September 2020 4,826 1 View

How to remove a non-ionic surfactant (eg-tween20) from the reaction mixture?

I attempted to prepare CuS nanomaterials using microemulsion method. I used tween20 as the surfactant. But found it is very difficult to remove the surfactant from microemulsion. How can we remove...

23 June 2020 5,758 17 View

How to measure Lowest Detection Limit of a sensor material in Electrochemical sensing?

I know Differential Pulse Voltametry is using to measure LOD value. But I do not know how to measure the Lowest Detection Limit of a sensor in Electrochemical sensing. Please reply. Thanks.

09 March 2020 3,676 3 View

What is the longest deletion or insertion that can be detected in next generation sequencing data analysis?

what is the longest deletion or insertion that can be detected in next generation sequencing data analysis

26 February 2020 3,113 3 View

What is the possibility of replicates being different from other replicates of same sample?

I have analyzed the dataset of GSE38132 from gene expression omnibus. The data is from cell line breast cancer ZR-75-1 which comprises of 9 conditions with 4 replicates for each condition making...

16 February 2020 5,954 4 View

How to perform normalization and calculate when there are multiple groups ?

I have 4 sample [control, pre-treatment, post-treatment, metastais] with 4 replicates which make total 4x4 = 16 samples I want to perform comparison of control with all other groups. For the said...

12 February 2020 6,528 2 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

31 July 2024 8,666 3 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

What exactly is RAG-LLM doing? Isn’t it data engineering?

What exactly is Retrieval Augmented Generation for Large Language Model doing? Isn’t it data engineering?

30 July 2024 7,376 3 View

May I know the exact Quartile of the journal- Advanced Engineering Materials (Wiley) for material science category?

In some data sources it has been grouped in Q1 and some shows it is Q2.

29 July 2024 4,227 2 View

Dmitry Zinovkin

Human protein atlas

https://www.proteinatlas.org/

Franziska Moll

In theory you can detect any protein by IHC if you have a working and specific antibody. Proteins that are secreted from the cell are very hard or impossible to detect. Here you would use RNAhybridisation to sudy at least gene expression.