Hi!

What kind of tissue culture you want to do? and which plant are you working on? depending on those, you can have a wide variety of tissues or plant parts to choose from. If it is micropropagation, then shoot tip would be a good choice. But, for tubers like potato u can use the newly germinating buds from the tuber (keep the tuber in dark for germination at RT). if you want to regenerate through calli formation then you have wide choices to select from depending on plants. Mostly for dicots, leaf tissue is a good choice. Tobacco, Arabidopsis, Potato, eggplant, tomato, different flowering plants, mostly they all form calli from leaf tissues and then new shoot germination can be initiated from it. for monocots, immature embroys could be a good choice. these can also be used for micropropagation (select the hormones accordingly).

Good luck

Hi!

What kind of tissue culture you want to do? and which plant are you working on? depending on those, you can have a wide variety of tissues or plant parts to choose from. If it is micropropagation, then shoot tip would be a good choice. But, for tubers like potato u can use the newly germinating buds from the tuber (keep the tuber in dark for germination at RT). if you want to regenerate through calli formation then you have wide choices to select from depending on plants. Mostly for dicots, leaf tissue is a good choice. Tobacco, Arabidopsis, Potato, eggplant, tomato, different flowering plants, mostly they all form calli from leaf tissues and then new shoot germination can be initiated from it. for monocots, immature embroys could be a good choice. these can also be used for micropropagation (select the hormones accordingly).

Good luck

It all depends on which plant species you are working. It is also depending on what do you want-- to propagate (for example, using the 'shoots' of a tobacco to propagate more plants quickly) or inducing callus (for examples, using hypocotyl of carrots or using cotyledons of cottons)......etc.

Simply, I think hypocotyl is one of the most active and easiest parts to culture. I used it a lot produce calluses and plantlets

Article Biochemical and Molecular Markers associated with callus ind...

the embryonic first leaves of a seeding

Depends on what you want - if it is mutiple shoots then definately shoot tip, nodal explant; if it is callus - leaf explant

I WILL RATHER PUT IT LIKE THIS: MOST FRESH PARTS OF PLANTS WILL HAVE MOST TOTIPOTENT CELLS AND HENCE LEADING FASTER DEDIFFERCIATION AND OR MULTIPLICATION SO TRY OUT THEM WHETHER IT SHOOT TIP OR ROOT TIP OR SEED GRWOEN PLANT LETS  EMBRYO FROM SEED TOO IS GOOD STARTING MATERIAL

For callus the leaf explant and for mutliple shoots the shoot tip or nodal explant  are the most preferred parts

use  meristamatic tissues from shoot for micropropagation & use root meristem cells for hairy root culture(for the production of secondary metabolites)

Shoot apex with younger leaves is most commonly used explant in almost all embryophytes irrespective of the objective of culture and this region provides physiologically young and active cells with genetic and metabolic homogeneity and leat microbiota.

Hello,

Choosing of explants generally includes juvenile parts of the plant like leaves, nodes. For somatic embryogenesis, anthers from unopened buds are used. Nodes and leaves are mostly used for callus induction, direct organogesis. If its for the enhancement of secondary metabolite production, you can find out the presence of  the particular metabolite in the particular part of  your plant of interest.

It depends of plant type and your aim - as the parts are more juvenile it will easier to obtain callus or to induce regenerating. Be careful with shoot tips if you want to induce undirect organogenesis because there are sleeping buds which could mislead you.

The choice of explant used for tissue culture depends on the type of experiment you are conducting. Generally healthy plant parts containing actively dividing (meristematic) cells are ideal. If you just need to multiply cells for a genetically stable progeny, any vegetative tissue with meristems are good. But for haploid culture you will need embryogenic tissue from anthers or ovaries. 

Dear Peers, 

Thanks a lot for your valuable suggestions and enhancing my knowledge.

Hi Arvind Kumar Goyal

Depend of course, the technique of tissue culture you will be used.

For incial micropropagation always are prefered use apical meristems because is the more cleaner and strong tissue with more speed growth.

If the technique for implanted to use will be another as callus culture, for obtened indifereciation tissuses, can be use leaf, stem, root. The big problems with this organs are the contamination and low growth, then you will need supplement the medium with more phytohormones or mutagenics, that which will lead to genetic variation and modification in the genotype of the donor.

What kind of tissue culture you want to do ? Whict plants are you you select and working on your Research work depending on variety ,type,composition and type of auxins & cytokinins majority of explants have best result belongs to meristamatic tissue of palts (shoot tip,nodal.internodal & root tips ) 

Different parts of plants used as ex plants in Micropropagation but which parts of plants made by Meristamatic which grow on nutrient madium eassy to develop multiple shoots or callus because any plant develops from embryonic tissue its made by Meristamatic tissu 

Suitablity of the explant vary depend on the purpose.For micropropagation nodal explant is the best starting material than shoot tip,root tip and internofal segments.

Well, as the others have indicated, explants with pre-existent buds/meristems such as shoot tips, nodal segments and cotyledon nodes will readily give multiple shoots with cytokinins such as BAP, zeatin etc. However, if you are looking for somatic embryogenesis or de novo organogenesis then you may find immature/mature zygotic embryos or cotyledons  useful. All the best!

I will like to corroborate what  Sagwa said,  the buds/meristems such as shoot tips, nodal segments and nodes from pre-existent  buds would readily give multiple shoots with cytokiniins such as BAP, zeatin etc. This is by far the best or most preferred type of explant for tissue culture.

i have done micropropagation using explants obtained from seedlings(in vitro raised).. what i want to know is "what is difference in using explants from in vitro seedlings compared to explants obtained from ex vitro raised mature trees.

Actually it depends on the goal of research. If the goal is for clonal propagation, the explant will be lateral or terminal bud or shoot. In case of callus induction, pieces of cotyledon, hypocotyl, stem, leaf or embryo may the choice depends on genotype.

you guys have references?

Can I use aril part from the fruits as the explant?

Its depend on the objective of tissue culture experiments. However, shoot tips and axillary buds are commonly used for micro-propagation.

Hi

You have to consider your project, because sometimes some explants are good for indirect shoot organogenesis, even we can see realtions between media culture and explants.

Greetings...

This depend on the your experiment project ,Some time leaf culture are more successively in Callus induced for Secondary metabolite production in compare with other explant ,in other hand, Shoot tip culture is very good sources for micropropagation approach.etc

Hi, based on an experiment I conducted, the performance of potato meristems obtained from different plant parts varried with the source of the meristems.

Hi, Answer of this question depended upon the purpose of the experiment and, I think that leafs is more attention in plant tissue culture .

It is basically dependent to your experiment. But most probably you can use young, juvenile and healthy explants such as axillary and apical meristems i.e. nodal segments and shoot tips for achieving high frequency early establishment of cultures under in vitro conditions.