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Questions related from Govind Kumar Rai
I want to present mean values (with SD/SE) of years of field data (agronomic, insect attack, protein expression, etc.) taken across 50 locations in three agro-climatic zone. What will me more...
06 June 2017 7,220 3 View
How a gene, responsible a target trait, is identified and isolated for further study....? Just the general steps.....
09 September 2014 6,975 2 View
Certain genes do not get expressed in 'control treatment' or 'control plants', and thus 'Ct' value of such target genes is not obtained in real-time expression experiments (Ct = 0). In such cases,...
04 April 2014 5,940 4 View
While calculation of the fold change expression of a transgene/gene, the expression level of target gene is normalize by subtracting delta Ct of treated sample with delta Ct of control sample....
03 March 2014 2,047 2 View
After precipitating DNA, if washing processed with water instead of 70% ethanol, then one will be able to recovered DNA immediately or it will get away with water. Do the DNA will get completely...
10 October 2013 9,408 9 View
How do we calculate expression level in a qRT-PCR in the case of transgenics (like expressing 'cry genes') where we do not have a calibrator control sample? The calibrator sample (Wild Type: WT or...
04 April 2013 9,687 3 View