Govind Kumar Rai

6 Questions 9 Answers 0 Followers

Questions related from Govind Kumar Rai

How to analyses data of 4 years in three different Agro-climatic zone. Pooled mean or mean of mean?

I want to present mean values (with SD/SE) of years of field data (agronomic, insect attack, protein expression, etc.) taken across 50 locations in three agro-climatic zone. What will me more...

06 June 2017 7,224 3 View

How to isolate a gene?

How a gene, responsible a target trait, is identified and isolated for further study....? Just the general steps.....

09 September 2014 6,976 2 View

How to calculate fold change expression of experiments where 'control treatment' or 'control plants' do not have any 'Ct' value of the target gene?

Certain genes do not get expressed in 'control treatment' or 'control plants', and thus 'Ct' value of such target genes is not obtained in real-time expression experiments (Ct = 0). In such cases,...

04 April 2014 5,941 4 View

Which plant should be used as control for normalization to calculate Fold Change Expression of a transgene in stressed transgenic plants?

While calculation of the fold change expression of a transgene/gene, the expression level of target gene is normalize by subtracting delta Ct of treated sample with delta Ct of control sample....

03 March 2014 2,048 2 View

What will happen if water is added to wash DNA pellet instead of 70% Ethanol? Do the DNA solubilization continues during immediate centrifugation?

After precipitating DNA, if washing processed with water instead of 70% ethanol, then one will be able to recovered DNA immediately or it will get away with water. Do the DNA will get completely...

10 October 2013 9,409 9 View

Real time PCR Expression Level Calculation

How do we calculate expression level in a qRT-PCR in the case of transgenics (like expressing 'cry genes') where we do not have a calibrator control sample? The calibrator sample (Wild Type: WT or...

04 April 2013 9,688 3 View