Like ompc, OmpA which outer membrane protein in e coli can be used to tag and express a larger gene like 1000bp?
I want to clone a gene in Pgemt vector having BamH1 frw enzyme and Sal1 frw enzyme. where as in Pgemt vector BamH1 is considered to be a enzyme which "Restriction Enzymes That Do Not Cut the...
10 November 2014 1,759 3 View
Can we use BL21D3 competent cell which was purchased from NEB as a starter culture stock? What I mean is, I want to use the chemically competent cell which was bought from NEB as a glycerol stock...
03 April 2014 2,300 3 View
In our lab we mainly work on E.coli. For the past 2 months we are getting contamination in our LB tubes (a thread like think is growing). We have contamination only in LB tubes, so we cleaned all...
02 March 2014 5,525 17 View
I have got my gene of interested in polyacrilamide gel. I want to elute that band and used that for further cloning, so please help in providing a protocol for elute my DNA in polyacrilamide gel.
10 November 2013 4,813 9 View
How to calculate 5*10^7 cells from bacteria which is having a particular OD after Induction...
10 November 2013 481 4 View
My gene of interest is 91bp. I cloned the PCR product in to Pgemt vector and digested with 2 enzymes. I am getting blue white colonies. While screening the white colonies, I am able to get the...
09 October 2013 6,796 9 View
How to run a polyacrylamide gel for DNA less than 100bp and what type of staining should be used? Alos, how to elude that gene of interest in that polyacrylamide gel?
09 October 2013 6,628 11 View
Seeking to amplify 2 genes: one is 441bp and another one is 88bp. The problem is that the annealing temp of the primer is 72degree.and the GC content of both frw and rev primers are 59.6 and 47.5...
08 September 2013 3,318 9 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...
03 March 2021 8,237 3 View
I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...
02 March 2021 6,270 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View