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Questions related from Murali Kannan
I want to clone a gene in Pgemt vector having BamH1 frw enzyme and Sal1 frw enzyme. where as in Pgemt vector BamH1 is considered to be a enzyme which "Restriction Enzymes That Do Not Cut the...
11 November 2014 1,866 3 View
Like ompc, OmpA which outer membrane protein in e coli can be used to tag and express a larger gene like 1000bp?
05 May 2014 1,696 0 View
Can we use BL21D3 competent cell which was purchased from NEB as a starter culture stock? What I mean is, I want to use the chemically competent cell which was bought from NEB as a glycerol stock...
04 April 2014 2,387 3 View
In our lab we mainly work on E.coli. For the past 2 months we are getting contamination in our LB tubes (a thread like think is growing). We have contamination only in LB tubes, so we cleaned all...
03 March 2014 5,768 17 View
How to calculate 5*10^7 cells from bacteria which is having a particular OD after Induction...
11 November 2013 548 4 View
I have got my gene of interested in polyacrilamide gel. I want to elute that band and used that for further cloning, so please help in providing a protocol for elute my DNA in polyacrilamide gel.
11 November 2013 4,882 9 View
How to run a polyacrylamide gel for DNA less than 100bp and what type of staining should be used? Alos, how to elude that gene of interest in that polyacrylamide gel?
10 October 2013 6,762 11 View
My gene of interest is 91bp. I cloned the PCR product in to Pgemt vector and digested with 2 enzymes. I am getting blue white colonies. While screening the white colonies, I am able to get the...
10 October 2013 6,866 9 View
Seeking to amplify 2 genes: one is 441bp and another one is 88bp. The problem is that the annealing temp of the primer is 72degree.and the GC content of both frw and rev primers are 59.6 and 47.5...
09 September 2013 3,379 9 View
