Can we use BL21d3 competent cell as a primary stock?

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What is this restriction digestion problem in Pgemt vector ?

I want to clone a gene in Pgemt vector having BamH1 frw enzyme and Sal1 frw enzyme. where as in Pgemt vector BamH1 is considered to be a enzyme which "Restriction Enzymes That Do Not Cut the...

10 November 2014 1,759 3 View

Which outer membrane proteins in e coli express larger proteins?

Like ompc, OmpA which outer membrane protein in e coli can be used to tag and express a larger gene like 1000bp?

04 May 2014 1,636 0 View

Can anyone help with contamination in our lab?

In our lab we mainly work on E.coli. For the past 2 months we are getting contamination in our LB tubes (a thread like think is growing). We have contamination only in LB tubes, so we cleaned all...

02 March 2014 5,525 17 View

How to elute a DNA from a polyacrilamide gel?

I have got my gene of interested in polyacrilamide gel. I want to elute that band and used that for further cloning, so please help in providing a protocol for elute my DNA in polyacrilamide gel.

10 November 2013 4,813 9 View

How to calculate 5*10^7 cells in RNA isolation

How to calculate 5*10^7 cells from bacteria which is having a particular OD after Induction...

10 November 2013 481 4 View

Can someone help with seeing band after restriction digestion?

My gene of interest is 91bp. I cloned the PCR product in to Pgemt vector and digested with 2 enzymes. I am getting blue white colonies. While screening the white colonies, I am able to get the...

09 October 2013 6,796 9 View

How to run a polyacrylamide gel for DNA of less bp(91bp)?

How to run a polyacrylamide gel for DNA less than 100bp and what type of staining should be used? Alos, how to elude that gene of interest in that polyacrylamide gel?

09 October 2013 6,628 11 View

Can someone advise on a problem in a PCR amplification?

Seeking to amplify 2 genes: one is 441bp and another one is 88bp. The problem is that the annealing temp of the primer is 72degree.and the GC content of both frw and rev primers are 59.6 and 47.5...

08 September 2013 3,318 9 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Aymeric Goyer

Yes, absolutely. You can prepare your own stock of chemically competent cell from the stock you purchased from NEB.

David Klaver

Yeah these are great.

As Aymeric said - you can grow a lot of stock for later use from the NEB cells.

Andrés González Rodríguez

Yes, you may use it without problems. When you need to grow it, just spread the glicerol on LB agar to obtain isolated colonies. In fact, I have used this method sometimes...