Actually, i would like to predict the transmembrane region in several membrane proteins. I have tried several online software's including TMpred, MEMSAT, PROTTER, HMMTOP, TMHMM and DAS. I have got different results from each software. For example i have predicted TM helix for protein WelM and got the results as follows

TMpred - 2 helices,

MEMSAT - 2 helices (Figure attached)

PROTTER - no helices (Figure attached),

HMMTOP- no helices,

TMHMM- no helices,

DAS- 3 helices

Im so confused about the results, anyone can suggest me the trustworthy software.

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