How to design guideRNAs that should target all the alleles of a gene in monocot?

Kumaravel Marimuthu @Kumaravel-Marimuthu

16 August 2022 1 5K Report

In banana, we are having multiple alleles. For distrusting the function of a gene through CRISPR/Cas9 it is important to mutate all the alleles through designing specific guideRNA repeats. How to precisely design guideRNAs that target all the alleles of a gene? If any, special tools is available , please specify.

Thanks

Kumaravel.M

Robert Adolf Brinzer

Your best option would be to align the sequences of all your alleles to look for conserved regions/domains and then design your gRNAs for those. For multiple sequence alignment I like to use MUSCLE but that is just my preference.

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