Marimuthu Anandharaj

10 Questions 12 Answers 0 Followers

Questions related from Marimuthu Anandharaj

Which online software is reliable for Protein Transmembrane helix prediction

Actually, i would like to predict the transmembrane region in several membrane proteins. I have tried several online software's including TMpred, MEMSAT, PROTTER, HMMTOP, TMHMM and DAS. I have got...

26 May 2017 6,590 4 View

Double band for a single protein on western blot?

Im working on yeast secretary protein, my protein was his tagged so i use Anti-his monoclonal antibody for WB, i can see the signal but i got two band on each protein (totally i have two proteins...

17 December 2015 5,457 12 View

How to select a correct primer for an unknown organism for PCR?

We isolated bacteria from various samples. It is identified by biochemical tests as Lactobacillus spp. Now we are going to sequencing it for species level identification. I'm a little confused...

30 March 2014 1,651 5 View

How to find a specific gene responsible for particular charecter from the 16S rRNA sequence?

I need to identify the gene responsible for production of bacteriocin, and cloned it into another organism but I don't know how to identify the gene from 16S rRNA sequence.

22 February 2014 2,430 2 View

Why do we perform coaggregation and autoaggregation assay for probiotic microorganism?

I want to know what is the need of these two test to select the potential probiotic strains..

28 January 2014 4,704 2 View

Is there any record of cholesterol reduction by Lactobacilus oris previously?

There are lots of studies which records the cholesterol reduction by Various Lactobacillus spp. but i want to know is there any researchers showing cholesterol reduction by Lactobacillus oris.

28 January 2014 6,509 1 View

How can we isolate chemical compounds from medicinal plants?

I want to know the method which is applied for isolating chemical compounds from plant materials.

19 January 2014 2,637 8 View

Anybody knows how Probiotic bacteria enter the breast of lactating mothers?

In earlier periods human milk was believed to be sterile and do not contain any microbial load. Nowadays plenty of research shows the presence of Probiotic microbes in human milk, I want to know...

19 January 2014 5,301 2 View

What is the most suitable media for the isolation of Weissella spp. from a fermented food source?

I have to isolate some lactic acid bacteria which includes Weissella spp. For lactobacillus, I used an MRS medium, but I don't think the same media can be used for Weissella spp.

03 January 2014 4,568 2 View

Can we use HPLC for the detection of specific enzymes?

I want to know if it is possible to identify specific enzymes using HPLC?

28 December 2013 493 2 View