Which one is best software for multiple sequence alignment and primer designing?

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How can i check primer degradation?

I have a constant issue of background in my assay. i am thinking its primer degradation, so how can i check that.

03 April 2018 8,851 12 View

What is Retention factor in densitometry?

I am doing densitometry on bands on agarose gel, i got band area, height, width, Rf value and adjusted RF value. I don't know what is meaning of Rf ( retention factor)? i run 2 samples in...

02 March 2018 9,845 1 View

How can i test pcr inhibitors in unknown sample?

I want to know that does my tick DNA contains pcr inhibitors or not?

31 December 2017 6,946 5 View

Lac Z and pUC 19?

Lac z is bigger in size as compared to pUC 19 plasmid how can we put lac z into pUC 19?

01 February 2017 6,745 2 View

In E.coli, mutation in Amber codon (UAG) suppression occur in tRNA genes that exist in multiple copies but in (UAA)& (UGA) 1 gene do this?why is so?

A non sense suppression is a suppression which is due to stop codon i.e,s codon get mutated and resulted in stop codon and truncated protein will produce then a nonsense suppressor will play the...

10 November 2016 3,926 1 View

I am using Unipro ugene (software) for Multiple sequence alignment .unable to get print of my result.Help needed ?

How to print the whole multiple sequence alignment from unipro ugene ? please find file in attachment.

01 January 1970 1,276 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View