Lac z is bigger in size as compared to pUC 19 plasmid how can we put lac z into pUC 19?
1. Why do you need to insert lac Z in pUC19? The plasmid contains "lac Z alpha" gene which could be used for blue-white selection too.
2. Normal plasmid like pUC19 should be able to take insert of size ~10kb or even more.
pUC19 does not contain an entire lacZ gene, but a sequence coding for a short, non-functional N-terminal segment denominated the alpha fragment. When expressed in a host expressing the remainder of the lacZ gene (an also non-functional segment denominated the omega fragment) both fragments associate in vivo to produce functional beta-galactosidase, a phenomenon known as alpha complementation.
If you wish to produce full-length lacZ in pUC19 you will have to amplify the missing lacZ fragment and clone it in-frame with the alfa fragment present in the vector.
Hope it helps!
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