What is Retention factor in densitometry?

03 March 2018 1 10K Report

I am doing densitometry on bands on agarose gel, i got band area, height, width, Rf value and adjusted RF value. I don't know what is meaning of Rf ( retention factor)?

i run 2 samples in triplicate and i want to run t test on densitometry data and want to look for p value, i am confused which values should i take from densitometry?

Kevin M. Carroll

What is the software you are using here?

To my knowledge, retention deals with the mobility of your protein. Basically- it deals with the distance traveled of the target protein from gel-to-gel or lane-to-lane. For the purposes of analyzing relative abundance, you would most likely want to focus on the 'area' measurement. The other measures could be useful to you for determining reliability of your runs.

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