Sammuel Shahzad

7 Questions 8 Answers 0 Followers

Questions related from Sammuel Shahzad

How can i check primer degradation?

I have a constant issue of background in my assay. i am thinking its primer degradation, so how can i check that.

04 April 2018 8,986 12 View

What is Retention factor in densitometry?

I am doing densitometry on bands on agarose gel, i got band area, height, width, Rf value and adjusted RF value. I don't know what is meaning of Rf ( retention factor)? i run 2 samples in...

03 March 2018 9,891 1 View

How can i test pcr inhibitors in unknown sample?

I want to know that does my tick DNA contains pcr inhibitors or not?

01 January 2018 7,005 5 View

Lac Z and pUC 19?

Lac z is bigger in size as compared to pUC 19 plasmid how can we put lac z into pUC 19?

02 February 2017 6,796 2 View

Which one is best software for multiple sequence alignment and primer designing?

i am using ugene but unable to get the pdf of alignment

01 January 2017 2,378 3 View

In E.coli, mutation in Amber codon (UAG) suppression occur in tRNA genes that exist in multiple copies but in (UAA)& (UGA) 1 gene do this?why is so?

A non sense suppression is a suppression which is due to stop codon i.e,s codon get mutated and resulted in stop codon and truncated protein will produce then a nonsense suppressor will play the...

11 November 2016 3,977 1 View

I am using Unipro ugene (software) for Multiple sequence alignment .unable to get print of my result.Help needed ?

How to print the whole multiple sequence alignment from unipro ugene ? please find file in attachment.

01 January 1970 1,345 0 View