I want to administer artificially synthesised microRNA in DMBA induced mammary carcinogenesis in mice. Generally for plant extract some researcher using ig. Which mode of administration (ig or ip or it or sc ) is most suitable for artificially synthesised miRNA in DMBA induced mammary carcinogenesis in mice ?
IG, IP or SC administration would result in delivery to compartments, from which miRNA would be very difficult to penetrate to mammary tissue, and so IV or IT would be more meaningful modes of administration for delivery to mammary gland tumors. Furthermore, systemic IV would be preferred over local IT administration, because IT would only allow local control of tumors. For IV administration, miRNA needs to be encapsulated in an appropriate delivery vehicle to allow its intracellular uptake. Several types of delivery vehicles are investigated (polymer-based, lipid-based), and some of them are also commercially available. Importantly, delivery of miRNA into cells in vivo is challenging and a much more complex problem than delivery of low-molecular biologically active compounds, which are typical components of plant extracts mentioned in this question.
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