I have been collecting breast tumor tissues from patients and am going to study the expression profiling of stem cell regulatory genes. Also, i would like to do assessment of breast cancer stem cells in tissue section by immunohistochemistry.
but am in little bit of confusion regarding markers. Which marker is best for assessment ? ( ALDH, CD133, CD24, CD44)
For the assessment of breast cancer stem cells, the best markers would be CD44, CD24, ALDH1, Lin, CD326 (EpCAM).
The level profiles of CD44high/CD24low/ALDHhigh or CD44high/CD24low/Lin-neg or CD44high/CD24low/EpCAMhigh would be the best evaluation of the cancer stem cell population.
For the assessment of breast cancer stem cells, the best markers would be CD44, CD24, ALDH1, Lin, CD326 (EpCAM).
The level profiles of CD44high/CD24low/ALDHhigh or CD44high/CD24low/Lin-neg or CD44high/CD24low/EpCAMhigh would be the best evaluation of the cancer stem cell population.
Hi there,
the answer by Amirali is very good.
If you need a more specific one, i would go for ALDH. It seems that in literature it is referred in a number of papers. In order to complement my answer i have attached here some references for you and also the profile of another researcher at Dana Farber who may be of help to you too.
https://www.researchgate.net/profile/Francisco_Beca
BW
Nikos
For the primary FACS selection, the markers which can be used CD45, ALDH1, and EpCAM.
CD45−/ALDH1+/EpCAM+ or CD45−/ALDH1+/EpCAM−.
I agree with Amirali Bukhari. The best markers for staining of breast cancer stem cells by immunohistochemistry is CD44, CD24. CD133 can be used for a lot of kind of cell lines by immunohistochemistry such as endothelial progenitor cells, so that, in my opinion, CD133 is not the best marker for breast cancer stem cells.
I do agree with Tam.. Coz CD133 has enigmatic function. Its not a specific cancer stem cell marker.
Especially EpCAM positivity should be taken into consideration along with CD44 positivity according to the clinical data.
There was a 2012 Astra-Zeneca slide presentation in a series of: 'Early Breast Cancer', first unit was about: 'Overview and assessment of Early Breast cancer', perhaps you can contact directly them, I can't send you the slides, because I'm not the author nor the copyright owner. Good day!
Hi,
I am looking to the CD24 and CD44 profile by FACS. I am using a breast cancer cell line: MCF10A ER-Src cells. Does anyone have a protocol for FACS staining for this purpose?
Thanks
Margarida Araújo
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