Paul David

2 Questions 10 Answers 0 Followers

Questions related from Paul David

What is the best way to do FACS staining, which includes both surface and intracellular staining together?

I want to stain human whole blood sample. I fix and permeabilise the cells with BD cytofix cytoperm for 10 mins. Wash them twice with 1X permwash buffer and then stain the cells with cocktail of...

07 July 2015 2,594 6 View

Is it possible to study about circulating tumor cells in HCC patients with the help of Flow cytometry. If yes, how much blood sample is required?

I have already standardized it with IF. want to do it with flow cytometry. please help me with the markers?

02 February 2015 3,784 2 View