Which markers are better for isolation of cancer stem cells?

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Do MDA MB 231 breast cancer cell lines change their morphology after several passages?

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Which is the best method for isolation of cancer stem cells?

Can anyone suggest the protocol for isolation of cancer stem cells from breast tumor tissues?

10 November 2014 2,125 5 View

How gene expression profile useful for clinicians?

How gene expression signature helpful for patient-tailored therapy strategies?

10 November 2014 6,518 1 View

How does hypoxia enhances stemness of cancer cells?

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Can any one provide reviews on tumor hypoxia and periostin gene function?

Dear all, can any one suggest me good literature on tumor hypoxia and its effects on periostin gene function in breast cancer.

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Which marker is best for staining of breast cancer stem cells by immunohistochemistry?

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How does hypoxia play an important role in the maintenance of a cancer stem cell niche?

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What are the emerging trends and opportunities for STEM education and careers in the online space?

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Paul David

CD133 has enigmatic function. I would suggest you to use CD44.

Roberto Würth

I suggest you CD44+/CD24-

Sandeep Rajput

Depends on the tumor type, cancer stem cell markers are different. For Breast its CD44+/CD24-/ESA+. For head and neck its CD24+/CD44+.

Steve Mcclellan

We have had very good sucess with CXCR4, MDR1 and ALDH (aldefluor)

Nick Asbrock

EMD Millipore has recently commercialized a red-shifted fluorescent substrate for aldehyde dehydrogenase (ALDH) that works in a similar fashion as ALDEFLUOR but allows the concurrent use of green fluorescent cell lines, antibodies, transgenic animals and reporter assays.

www.emdmillipore.com/aldered

Attached is the Nature publication describing the AldeRed reagent