Which kit is good for isolation of circulating tumor DNA (ctDNA) from blood?

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Drugs inhibiting pAkt but not pmTOR?

Hello All, Recently we found an interesting observations on cellular signalling events after some drug assays in breast cancer cell lines. The drug seems to be hitting pAkt, however no effect on...

14 March 2024 6,411 0 View

SNU398 cells morphology?

Hi I am culturing SNU398 cells and over the period of time that it displays a two different kind of morphology (one is typical Polygonal kind and the other one is more rounded-like). Do you think...

09 October 2023 8,099 0 View

Intensity changes with probe - ligand complex vs probe only in FP assay?

I am using a chemical tagged with fluorophore for FP assay. The chemical is a known binder for the protein. After optimizing G-factor to 0.91 for improving the FP values, I observed in subsequent...

02 April 2023 3,955 5 View

Can racemic mixture behave different than components in a fluorophore biophysical assay? Can it be more active than pure fractions?

Biophysical assay involves cleaving a quenched fluoregenic substrate that results in increased fluorescence. Difference between racemic mixture and pure components came to be ten times different...

23 January 2023 9,723 1 View

In case of screening for inhibitors for an enzyme, is it important to pre-incubate the compounds with enzyme before adding the substrate? why?

I am used to pre-incubate the test compounds with enzyme for durations ranging from 15 min to 45 min before adding the substrate and initiating the reactions. However, in process of designing a...

12 December 2022 3,661 2 View

Storage temperature of lentivirus particles ?

Hi everyone I just wanted to ask if anyone stored lentivirus particles at -20C. I read that at temperature of -80 they are stable for upto 1-2 years. Does storing them at -20 loose their...

28 July 2022 4,708 4 View

How tvt is transferred to human being?

How TVT is transmitted to human being. And many chances of getting recovered from this in case of female patients?

04 June 2022 1,505 1 View

MCF 10A Media?

Dear All I am growing MCF10 A cells in the ATCC recommended media (DMEM: F12, 5%Horse serum, etc). I was just wondering if anyone has used the DMEM (or RPMI) with 10% FBS for the same cells? If...

20 December 2021 685 0 View

For Denaturing Gradient Gel electrophoresis analysis (DGGE), where should gc-clamp to be added... Forward primer or reverse primer?

For my research work on bacterial fingerprinting, I have to run DGGE analysis. I have to design my primers and for DGGE I need to add gc-clamp to primers. Some articles says gc-clamp to be added...

31 October 2021 6,938 1 View

What should be done to remove background in the negative lane (IgG). Should the IP be done overnight or for short duration?

I am getting a high background in the negative control (Normal secondary IgG) lane. The protocol is the lysate (about 500ug) is mixed with capture antibody and negative antibody overnight at 4C....

06 October 2021 691 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

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05 August 2024 9,059 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Sandeep RAJ R

QIAamp Circulating Nucleic Acid Kit

Gaurav Kumar

We are using Qiagen and it is working best in my lab system ..

Vikrant Palande

Thanks Sandeep and Gaurav..

Gaurav how do you process the blood for ctDNA isolation? If you use plasma, then when do you process it after collection and how do you store the plasma until it is processed for isolation of ct DNA?

Thank you