Hello All,
Recently we found an interesting observations on cellular signalling events after some drug assays in breast cancer cell lines. The drug seems to be hitting pAkt, however no effect on pmTOR and its substrate (pS6 and p70S6k) was seen. Moreover, they didn't observe much impact on p-ERK as well, however their cell viability data suggests a significant growth inhibition. Have you ever come across such observation? Is it possible that after hitting pAkt the drug may be targetting other Akt substrates like GSK or Foxo to inhibit cellular growth?
Thanks