How the values of errors can be calculated for thermodynamic data?

I have calculated thermodynamic parameters for surfactant solutions in aqueous and mixed alcoholic solvents. I want to calculate the value of errors in the data and represent it in the table along...

22 February 2021 239 2 View

Which vegetable crops can tolerate salinity , Except Solanecious crops ?

Except ( Tomato-Brinjal-Capscum-Chilli) .

27 December 2020 8,362 12 View

Can anyone put up some light on how added urea could be detected from food item(not milk)?

We doubt that some people add urea in fish based food item, any one can suggest some method by which we can detect the uear qualitative and quantitativly.

16 November 2020 6,839 3 View

How to determine concentration of each solute in a solution?

I have 3 drugs mixed in a solution coming out from a microfluidic device. I know the concentration of each drugs at inlet. If I have absorbance data of each inlet drug solutions, how to estimate...

13 October 2020 898 3 View

Breast Cancer Immunohistochemistry (IHC) slides analysis?

I want to know how to look/analyze different parts in a tumor section like stroma, Ductal epithelial, epithelial lining, luminal epithelial etc etc in a breast cancer tissue slide. Also is there...

02 June 2020 5,050 3 View

Semi Dry vs Wet Transfer recipe?

Hi I found that there are two different recipes for transfer depending on the mode (Semi-dry or wet) of the transfer. Why is it so? If anybody has prior experience with the use of both the...

26 December 2019 946 3 View

How to avoid the interference signals arising because of the wires in Digital oscilloscope?

I am trying to make pressure/force sensors and measuring the corresponding signals on digital oscilloscope (DSO). But most of the time observing the interfering signals because of the wires/clips...

27 November 2019 3,169 5 View

Double delta Ct values given by PCR machine according to Applied biosystems Real Time system?

I performed real-time PCR using an Applied biosystems machine. The double delta Ct values ( according to the machine) for the gene of interest after certain treatments were given as -5.12454;...

23 November 2019 5,286 3 View

What would be the correct method for exploring factors, reliability & validity?

I have designed a scale where resposndents have to choose the most preferred response and the least preferred response. Most preferred response will be given 2 weightage while least preferred...

12 November 2019 8,197 1 View

What is (dissolved) oxygen concentration found in cell culture media?

Hi, I wanted to know concentration of (dissolved) oxygen in (mg/L) in cell culture experiments in standard incubator condition. Your help will be very much appreciated. Thank you.

04 August 2019 8,131 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

No title

02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View