Biophysical assay involves cleaving a quenched fluoregenic substrate that results in increased fluorescence. Difference between racemic mixture and pure components came to be ten times different in terms of IC50!
Adam B Shapiro
I'm assuming that you are asking about inhibitors of an enzyme. If the mixture of enantiomers is truly racemic (50:50), then the maximal differential potency between the racemic mixture and the more active of the purified enantiomers is 2-fold, since the racemic mixture is 50% active enantiomer. The differential between the more active and less active enantiomer can be much larger, since one enantiomer can, in principle, be completely inactive.
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