For PCR purposes, the kit for genomic DNA kit, is generally capable to produce adequate results for both plasmid and the genomic DNA in gram-negative bacteria. However, you can even omit the kit method, simply boil and lyse the bacteria culture [100 oC for 5 min] and take 1-2 of the supernatant as a DNA template to perform PCR. It is generally works well with small amplicon (for example,
For PCR purposes, the kit for genomic DNA kit, is generally capable to produce adequate results for both plasmid and the genomic DNA in gram-negative bacteria. However, you can even omit the kit method, simply boil and lyse the bacteria culture [100 oC for 5 min] and take 1-2 of the supernatant as a DNA template to perform PCR. It is generally works well with small amplicon (for example,
Mona Barakat There is a lot, I suppose. We use a spin column from Omega.
any kit should work depends on your application .. alcaline lysis (no kit) works good for re analyses and cloning boiling method works for pcr and clone screening
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