Kiran Sreeja Jayan

4 Questions 38 Answers 0 Followers

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Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

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His- Protein precipitation during buffer exchange?

Hi all, I am having issues with protein precipitation during buffer exchange. His-tag protein is eluted in 20mM HEPES, 200mM imidazole, 500mM NaCl at pH8.0. During elution, the tube with the...

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Protein precipitation during buffer exchange?

Hi all, I noticed small white precipitates formed during buffer exchange using Vivaspin. I am new to protein biology. I am not sure if the white stuff that I see are protein precipitates or...

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Can I use DMINDA for scanning whole genome sequence of a prokaryote to identify transcription factor binding sites?

Hi, I am not very good at computational biology. I am working on a transcriptional repressor protein which is believed to be associated with virulence. I did DNAse 1 footprinting to determine the...

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