4 Questions 41 Answers 0 Followers
Questions related from Kiran Sreeja Jayan
Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...
03 February 2021 2,831 7 View
Hi all, I am having issues with protein precipitation during buffer exchange. His-tag protein is eluted in 20mM HEPES, 200mM imidazole, 500mM NaCl at pH8.0. During elution, the tube with the...
11 June 2017 2,527 11 View
Hi all, I noticed small white precipitates formed during buffer exchange using Vivaspin. I am new to protein biology. I am not sure if the white stuff that I see are protein precipitates or...
30 August 2016 4,386 9 View
Hi, I am not very good at computational biology. I am working on a transcriptional repressor protein which is believed to be associated with virulence. I did DNAse 1 footprinting to determine the...
31 July 2016 6,020 3 View