How to solve early solidification which causes hanging web in electrospinning?

I want to make electrospinning scaffold with PLA/PCL/PMMA/Collagen with these methods: 1. I use chloroform:methanol 3:1 to dissolve PLA (mW 80.000, 2 wt%), PCL (mW 70.000, 3 wt%), and PMMA mW...

01 November 2019 6,028 5 View

How can I solve the needle clogging problem during electrospinning with PLA/PCL/PMMA/Collagen?

For my thesis research, I dissolved PLA and PCL in chloroform: methanol (3:1), PMMA (350.000 MW, 13 wt%) in acetone, and collagen in acetate acid--and then, I mixed them together. The problem is...

06 October 2019 8,311 4 View

How long does it take to mix PMMA with PLA/PCL?

For my thesis research, first, I mix PLA (solvent: chloroform/methanol) and PCL (solvent: chloroform/methanol) for 2-3 hours. After that, I want to mix PLA/PCL with PMMA (mW 350.000 solvent: ethyl...

02 October 2019 7,185 1 View

Which is the best way to disrupt and prepare renal tumor tissue for flow cytometry?

I mechanically disrupted renal tumor tissue and then froze it at -70 for 1 month in DMSO 10% (in RPMI). Then samples were thawed at room temperature, centrifuged, resuspended in flow cytometry...

26 August 2013 1,362 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View