Evangelina Almada

2 Questions 1 Answers 0 Followers

Questions related from Evangelina Almada

Which is the best way to disrupt and prepare renal tumor tissue for flow cytometry?

I mechanically disrupted renal tumor tissue and then froze it at -70 for 1 month in DMSO 10% (in RPMI). Then samples were thawed at room temperature, centrifuged, resuspended in flow cytometry...

27 August 2013 1,445 2 View

Which is the best way to process renal tumor tissue to do flow cytometry?

I tried it with frozen tumor cells that I've got from mechanical disruption and filtration, but i can only sea dead cells.

26 August 2013 8,358 1 View