Evangelina Almada

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Questions related from Evangelina Almada

Which is the best way to disrupt and prepare renal tumor tissue for flow cytometry?

I mechanically disrupted renal tumor tissue and then froze it at -70 for 1 month in DMSO 10% (in RPMI). Then samples were thawed at room temperature, centrifuged, resuspended in flow cytometry...

27 August 2013 1,430 2 View

Which is the best way to process renal tumor tissue to do flow cytometry?

I tried it with frozen tumor cells that I've got from mechanical disruption and filtration, but i can only sea dead cells.

26 August 2013 8,344 1 View