Do DMF, NMP, and DMSO dissolve in PMMA Nanosphere?

01 January 1970 4,833 1 View

How can I separate polymer nanoparticles from the ethylene glycol?

01 January 1970 2,785 8 View

What are the dispersion medium and solvents of PMMA?

I prepared a PMMA nano-sphere and I want to disperse it in polymer matrix. I found that it disperses in water, but it gives a clear solution in DMSO. Does this mean that it dissolves in DMSO? I...

01 January 1970 1,321 11 View

How can I exactly determine the type of Cu2O semiconductor through the mott-shottcky?

What is the instrument and the conditions required to to this measurement? Thank you.

01 January 1970 9,397 1 View

How to eliminate the thickness effect ?

01 January 1970 6,320 3 View

What is the dispersion medium and the solvents of PMMA ?

01 January 1970 2,429 0 View

How can I determine the mott-schottky using potentiostat work station?

I prepared n type semiconductor using electrochemical method and I need to determine the photochemical properties.

01 January 1970 6,237 1 View

Does the electrical resistance of the substrate have an effect on the morphology of the deposited film?

01 January 1970 383 12 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View

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17 February 2021 4,217 3 View