Which is the best software to design nested PCR primers?

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On what basis you choose Mass spec %Cov(95) cutoff score to validate a protein through CoIP?

I am working on centrosomal proteins. I overexpressed my POI and then I did SNAP capture pulldown followed by mass spec and I got approximately 700 protein hits. I am trying to validate specific...

03 July 2024 5,701 0 View

P-nitrophenol acetate assay for esterase and media supernant colour interference. what can be done?

I am performing p nitrophenol acetate assay for esterase enzyme. My media contains a different concentration of peptone (0.5, 2, 5%) and after centrifugation the supernatant is slight yellow....

16 June 2024 4,381 0 View

Has anyone used starbase or lncBase to predict the interactions between miRNA and lncRNA?

Softwares used to determine the interactions or binding sites on lncRNA

28 April 2024 2,745 0 View

Can anyone recommend a company that does LC-MS/MS for silver stained gels where I can outsource my samples for proteomics analysis?

Looking for most cost-effective manner to outsource my samples (silver stained gels) for proteomics analysis using LC-MS/MS. If anyone has outsourced samples outside or in INDIA for LC-MS/MS and...

21 April 2024 309 2 View

Can anyone help me in functional annotation of lncRNAs? Or if someone has worked with NONCODE please help?

NONCODE is the database used for the different kind of information related to the lncRNAs like disease involvement, orthologs etc.

09 March 2024 7,571 0 View

What could be the cause of getting such blots?

my protein is a nucleolar protein which is tagged with HA, FLAG and myc tag. i have over-expressed it and good GFP expression was observed which is directly proportional to the amount of my...

11 January 2024 2,057 2 View

What is new in Menstrual Health and Hygiene in terms of marketing?

I am researching the taboo behavior around women's menstrual health and hygiene, and exploring ways to shift it towards marketing through better research design.

16 December 2023 2,530 1 View

When I published my article to a journal, it was not Scopus indexed. Now the journal is indexed in Scopus. Will my paper also be indexed now?

The paper was published one year back in a peer-reviewed DOAJ indexed journal by Elsevier. Now the journal has been indexed in Scopus. Will my previously published article in that journal be also...

11 December 2023 4,932 4 View

What are R packages for detecting peaks and peaks area for HPLC chromatogram?

I am performing snake venom fractionation using Reverse-phase HPLC. It generates multiple peaks with different area under the curve. Since I'll be analyzing multiple chromatograms at different...

08 November 2023 9,134 0 View

Anyone working on lncRNAs? If anyone knows about the screening of the lncRNAs using bioinformatics if pathway is already known?

I already know the pathway but want to know the upstream lncRNAs that regulates that pathway using the datasets and bioinformatics.

08 October 2023 2,798 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View