Can anyone recommend a company that does LC-MS/MS for silver stained gels where I can outsource my samples for proteomics analysis?

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P-nitrophenol acetate assay for esterase and media supernant colour interference. what can be done?

I am performing p nitrophenol acetate assay for esterase enzyme. My media contains a different concentration of peptone (0.5, 2, 5%) and after centrifugation the supernatant is slight yellow....

16 June 2024 4,381 0 View

How to calculate dosage for cell culture treatment by using plant extract ?

how to calculate dosage for cell culture treatment by using plant extract ?

12 May 2024 7,335 1 View

How to check whether the qPCR primers degraded or not?

I had transformed my desired gene along with selectable marker into rice plants and started with gene expression studies. Previously Amplification was observed in both genes but now on repetition,...

14 March 2024 7,841 1 View

Which software is the best for studying root architecture in plants?

Hi, Is there any free application I can use to study root morphology? The following information would be helpful to me: primary and lateral root lengths, root density, and root area. Thank you

11 January 2024 6,401 2 View

What could be the cause of getting such blots?

my protein is a nucleolar protein which is tagged with HA, FLAG and myc tag. i have over-expressed it and good GFP expression was observed which is directly proportional to the amount of my...

11 January 2024 2,057 2 View

What is new in Menstrual Health and Hygiene in terms of marketing?

I am researching the taboo behavior around women's menstrual health and hygiene, and exploring ways to shift it towards marketing through better research design.

16 December 2023 2,530 1 View

When I published my article to a journal, it was not Scopus indexed. Now the journal is indexed in Scopus. Will my paper also be indexed now?

The paper was published one year back in a peer-reviewed DOAJ indexed journal by Elsevier. Now the journal has been indexed in Scopus. Will my previously published article in that journal be also...

11 December 2023 4,932 4 View

Why C4 minor millets, even under good management do not out yield C3 rice ?

Despite having negligible photorespiration and photosynthetic advantages, what are the factors or reasons that C4 minor millets failed to surpass the yield of C3 rice ?

10 December 2023 702 1 View

What are R packages for detecting peaks and peaks area for HPLC chromatogram?

I am performing snake venom fractionation using Reverse-phase HPLC. It generates multiple peaks with different area under the curve. Since I'll be analyzing multiple chromatograms at different...

08 November 2023 9,134 0 View

How to obtain permission for translation of scale?

How to obtain the permission from original author for translation of scale?

27 September 2023 7,521 2 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View