Himanshi Bhatia

5 Questions 10 Answers 0 Followers

Questions related from Himanshi Bhatia

Interaction between lncRNAs and miRNAs

How do lncRNAs act as miRNA decoys/sponges? Do they only bind to the miRNA seed sequence or can they act by binding to other parts of the miRNA as well? Is it possible for lncRNA to act at...

22 June 2017 1,615 3 View

Which is the best software to design nested PCR primers?

I have to perform 5' RLM-RACE for target validation of chickpea miRNAs. Which primer design softwares have the option for designing primers for nested PCR?

05 July 2016 3,331 4 View

How to detect pre-miRNA levels in plants?

I want to detect the the levels of precursor miRNAs. Although there are many studies that explain detection of pre-miRNAs in animals, I have not been able to find many examples of the same in plants.

09 April 2016 9,112 2 View

Is it advisable to embed liver sections in paraffin for lipid staining ?

I need to determine lipid accumulation in liver. I plan to fix the tissue in 10% formalin. Should I embed the fixed tissue in paraffin, or cryopreserve the fixed tissue to prepare sections ?

10 November 2014 1,776 9 View

Has anyone used Fermentas RT enzyme ep0441?

We use fermentas RT enzyme (catalog number - ep0441) for reverse transcription. I was wondering if the cDNA formed is single-stranded or double stranded, as the enzyme has RNA-dependent and...

16 July 2014 2,851 1 View