Himanshi Bhatia

4 Questions 8 Answers 0 Followers

Questions related from Himanshi Bhatia

Which is the best software to design nested PCR primers?

I have to perform 5' RLM-RACE for target validation of chickpea miRNAs. Which primer design softwares have the option for designing primers for nested PCR?

05 July 2016 3,269 4 View

How to detect pre-miRNA levels in plants?

I want to detect the the levels of precursor miRNAs. Although there are many studies that explain detection of pre-miRNAs in animals, I have not been able to find many examples of the same in plants.

09 April 2016 9,040 2 View

Is it advisable to embed liver sections in paraffin for lipid staining ?

I need to determine lipid accumulation in liver. I plan to fix the tissue in 10% formalin. Should I embed the fixed tissue in paraffin, or cryopreserve the fixed tissue to prepare sections ?

10 November 2014 1,693 9 View

Has anyone used Fermentas RT enzyme ep0441?

We use fermentas RT enzyme (catalog number - ep0441) for reverse transcription. I was wondering if the cDNA formed is single-stranded or double stranded, as the enzyme has RNA-dependent and...

16 July 2014 2,785 1 View