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Questions related from Himanshi Bhatia
How do lncRNAs act as miRNA decoys/sponges? Do they only bind to the miRNA seed sequence or can they act by binding to other parts of the miRNA as well? Is it possible for lncRNA to act at...
22 June 2017 1,611 3 View
I have to perform 5' RLM-RACE for target validation of chickpea miRNAs. Which primer design softwares have the option for designing primers for nested PCR?
05 July 2016 3,327 4 View
I want to detect the the levels of precursor miRNAs. Although there are many studies that explain detection of pre-miRNAs in animals, I have not been able to find many examples of the same in plants.
09 April 2016 9,108 2 View
I need to determine lipid accumulation in liver. I plan to fix the tissue in 10% formalin. Should I embed the fixed tissue in paraffin, or cryopreserve the fixed tissue to prepare sections ?
10 November 2014 1,767 9 View
We use fermentas RT enzyme (catalog number - ep0441) for reverse transcription. I was wondering if the cDNA formed is single-stranded or double stranded, as the enzyme has RNA-dependent and...
16 July 2014 2,842 1 View