I need to culture Ecoli, Enterococcus, and Lactobacillus strains in a minimal medium with reduced carbon sources in an anaerobic environment. I tried culturing with the M9 medium but the growth is very very slow. The Ecoli inoculated with 0.1OD took nearly 24hrs to reach an OD of 0.5 whereas the Lactobacillus and Enterococcus could not even reach the 0.2 OD. Could anyone suggest me a better minimal media which is appropriate for the anaerobic bacterial culturing.
Thanks in advance.
Lactobacillus is fastidious bacteria. So I suggest to use MRS L- cysteine.HCl medium. It's modified MRS broth medium by adding L- cysteine.HCl to be anaerobic medium. It's a rich media you can use it for others species. Or you can use modified Nutrient broth media by adding sucrose (as a carbon source) and L - cysteine.HCl (as an anaerobic factor).
For lactobacillus, I would suggest to use MRS with L-cysteine hydrochloride, which is commonly used to culture anaerobically. For Ecoli, maybe you can use EMB. For anaerobic culture, maybe you can degas the media before you proceed culture, and if can, culture them in an anaerobic chamber.
I have the same problem. My main concern is how to find a minimal medium for lactic acid bacteria to test their ability to utilise carbon (and nitrogen). By definition, a minimal medium cannot contain a chemically undefined component. Using MRS will complicate my analysis because it includes chemically-undefined ingredients such as beef and yeast extracts, which have carbon sources. After a quick search, the only paper I found to provide a minimal medium for lactic acid bacteria, all containing chemically-defined components is this:
Development of a minimal growth medium for Lactobacillus plantarum - Wegkamp - 2010 - Letters in Applied Microbiology - Wiley Online Library
Article Development of a minimal growth medium for Lactobacillus plantarum
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