I need to measure the urea level in the cell culture system as well as culture media?
Any recommendations?
Alexandr Chernov
Using serial dilution, prepare the following urea/nitrogen standards in hepatocyte culture media: 200, 100, 50, 25, 12.5, and 0 µg/mL. Add 10 µL of standard or sample to each well of a 96-well plate. Mix 5 mL of BUN color reagent with 10 mL of BUN acid reagent (see Subheading 2.6.6). Add 150 µL of the BUN reagent mixture into each well. Cover with adhesive plate sealer and incubate at 60°C for 90 min. Cool plate on ice for 5–10 min before reading. Read plate absorbance at 520 nm.
http://www.altex.ch/resources/altex_2009_4_295_302_Ullrich.pdf
Urea Quantification
Article Long-Term Culture and Coculture of Primary Rat and Human Hepatocytes
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