The duration and depth of anesthesia is variable widely .A prolonged anesthesia may increase contact time of the TNBS solution with the colon. I did it by Ether ,but it is hard to control .So which is the ideally anesthesia ?intraperitoneal anesthesia or inhalation anesthesia? I know it may be Continuous Inhalation Anesthesia.because the anesthesia machine is not access to use for me.in addtion,if the mice can not be anesthesiaed ,how to avoid the mice quickly excrete the TNBS solution?
* GUIDELINES - PREPARATION OF KETAMINE/XYLAZINE COCKTAIL FOR MICE
Use of a sterile injection vial is required (e.g. redtop blood collection tube; commercial injection vial)
Mixing instructions:Verify the concentration of your drugs prior to mixing
For a 10mL vial using ketamine 100 mg/mL and xylazine 100 mg/mL add:1.75mL ketamine (100 mg/mL)
0.25 mL xylazine (100 mg/mL)
8 mL saline or sterile water for injection
Use of the following template for a label is recommended:Mouse Anesthetic Mix: Ketamine/Xylazine
Dosage: 0.1 ml/ 20gm IP
Delivers: 87.5 mg/kg Ketamine/12.5 mg/kg Xylazine
Concentration: 17.5 mg/mL Ketamine/2.5 mg/mL Xylazine
http://animal.research.uiowa.edu/iacuc-guidelines-anesthesia
or
Ketamine/Xylazine
The Ketamine/Xylazine combination is considered to be a very reliable anesthetic for mouse surgery. The recommended dose varies: It is 100-200 mg/kg body weight for Ketamine and 5-16 mg/kg body weight for Xylazine injected intraperitoneally. Although some labs use Ketamine at a dose of 35-50 mg/kg body weight, Ketamine at 50 mg/kg combined with 10 mg/kg Xylazine does not produce a consistent reliable level of immobilization or anes¬thesia in Syrian hamsters, and a dose of 150 mg/kg Ketamine combined with 10 mg/kg Xylazine has been suggested).
The most widely used dose of Ketamine/Xylazine for mouse surgery is 100 mg/kg and 10 mg/kg body weight, respectively (Flecknell 1993). Duration of the effect may be extended by increasing the proportion of Xylazine or by an additional dose of Ketamine.
Premedication with anticholinergic drugs such as atropine (0.02-0.05 mg/kg body weight) is often used to prevent bradycardia (slow heart rate) caused by Xylazine, and to control excessive bronchial and salivary secretions caused by Ketamine (Nowrouzian 1981; Magoon et al. 1988).
The recipe below provides an onset of 3-5 minutes with 30-40 minutes dura
tion of surgical anesthesia.
Ketamine (50 mg/ml) (Vetalar, Ketaset, Ketalar) 2 ml (100 mg)
Xylazine (20 mg/ml) (Rompun) 0.8 ml (16 mg)
Water (sterile) to 10 ml
Store at 4°C for a maximum of 2 weeks. Inject 0.1 ml per 10 grams of body weight (100 mg/kg Ketamine, 16 mg/kg Xylazine).
http://cshprotocols.cshlp.org/content/2006/1/pdb.rec702.full?text_only=true
Hi Xinjun,
We did this model by isoflurane or sevoflurane. The anesthesia duration & depth is easy to control.
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