Chen Xin Jun

10 Questions 6 Answers 0 Followers

Questions related from Chen Xin Jun

Is it necessary to match the mice by body weight in TNBS-induced mice colitis?

the average weight of BALB/c mice is about 20g, but in some groups the weight is not homogeneous，for example the mini is 18g ,the max is 23g.in order to reduce that disparity.Should I match the...

04 April 2015 3,190 4 View

Which is the best anaesthesia method used in TNBS-induced BALB/c mice colitis?

The duration and depth of anesthesia is variable widely .A prolonged anesthesia may increase contact time of the TNBS solution with the colon. I did it by Ether ,but it is hard to control .So...

04 April 2015 6,360 3 View

How do I draw a distinction between E coli and citrobacter rodentium？

Both can develop pink colony on MacConkey agar.Does someone have a simple and effective techniques for differentiation? If I want to count the number of citrobacter rodentium in feces, how can I...

09 September 2014 911 5 View

What is wrong with my Citrobacter rodentium -induced colitis?

Citrobacter rodentium were grown overnight in LB broth ,after centrifugation ,the pellet were resuspended with PBS.BABL/C mice were inoculated by oral gavage (approximately 2X10^8 CFU). But after...

08 August 2014 2,973 5 View

What is the difference between PBS and physiological saline ?

I‘m going to prepare yeast suspension of saccharomyces boulardii, and a group of mice will be given about 10^9 CFU/ml by oral administration every day. I would like to know, which solution to...

07 July 2014 2,245 16 View

How long can yeast suspension keep in 4℃？

I‘m going to prepare yeast suspension of saccharomyces boulardii,and a group of mouse will be given about 10^9 CFU/ml by oral administration every day. I would like to know how long can yeast...

07 July 2014 9,543 8 View

Should I use female or male BALB/c mice?

I am trying to establish BALB/c mouse colitis induced by citrobacter rodentium. Iused flow cytometry to analyze the Treg cell of LPMC from inflamed colon. I would like to know, based on...

07 July 2014 6,841 5 View

How do I exactly analyze the Th17/Treg balance in colitis model?

I am going to evaluate the preventive effect of probiotic in C.rodentium induced colitis. Especially I want to test its ability in restoring the imbalance of Th17/Treg. First, I will use flow...

06 June 2014 6,262 3 View

What is difference between staining buffer and FACS buffer?

Before surface and intracellular staining, should I choose which solution to wash cells? Can I use PBS? In FACS buffer, what is quantity of FBS? Is the bigger the quantity, the better?

06 June 2014 4,709 6 View

Can I skip the step of stimulation before surface staining for analysis Treg cell use flow cytometry?

I just try to analysis the Th17/Treg imbalance from isolated LPMC use flow cytometry. For analysis of Th17, cell activation ( stimulated with PMA and ionomycin) is often required. But for analysis...

06 June 2014 926 4 View