the average weight of BALB/c mice is about 20g, but in some groups the weight is not homogeneous，for example the mini is 18g ,the max is 23g.in order to reduce that disparity.Should I match the mice by body weight ? if I put the 18g or 23g around in each group? I wonder whether it will have a influence on the colitis model ?
Daniel Seidel,thank you for answering.by the way,how to avoid the mice quickly excrete the TNBS solution.? is it necessary to anesthesia the mice? I did it by Ether ,but it is hard to control ,If the mice anesthesia deep,it goes bad.so I would like to ask that which anesthesia method is suitable for BaBl/c mice？
Jun,
The randomization group is very important in vivo studies. Please do not put small mice or bigger mice into control group.
thank you for all the answering.an other question: although the total TNBS is 1mg. is there any difference between 100ul 1mg/50 %ethanol and 40ul 2.5mg/50%ethanol TNBS solution (TNBS:5%w/v stock solution) ?
If you randomize the mice the can have different masses of the body to the control or experimental group, but if they all are from one patch of mise from the same nursary it will not too important .