Citrobacter rodentium were grown overnight in LB broth ,after centrifugation ,the pellet were resuspended with PBS.BABL/C mice were inoculated by oral gavage (approximately 2X10^8 CFU).
But after one week ,the mice show no significant body weight loss,however, in some article the authors reported that Cr-infected mice demonstrated significant weight loss by day2-day3 postinfection.So I would like to know whether the rapid loss of body weight is a overt clinical sign of infection? and is 2X10^8 CFU enough to induced the colitis ?should I increase the concentration?
Hi Chen, the answer can be simple. It could be the CR strain used, but most importantly, BALB/c mice show resistance to CR. Better use C57BL/6 or C3H/HeJ. Read this paper
I guess the rest of my answer doesnt show...you should plate the feces every day on macconkey agar to show if CR is passing through the gut as well
Yes BALB/c is resistant to CR infection try to increase the no of bacteria for inoculation.
But if you want to study CR induced colitis use C57BL/6 mice.
Yes there have been some reports of Balbc mice being resistant to Citrobacter infection. You could increase the dose but the best way to determine if your mice are infected is to plate homogenized feces onto selective media plate to determine the shedding kinetics. Also the microflora of the mouse colony in that particular mouse room could be very competitive with Citrobacter.
Hello Chen,
I am using the same model in black B6 mice and I am infecting the mice with 1x10^9 cfu/ml and do not see the death of the mice and not even significant body weight loss. I assume it has to be the institution to institution variation in the mice immune system and microbiome which might be helping the mice to survive and remove the CR effectively.
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