which is bette; Luminex 200 thermo or Bio-Plex 200 Biorad? If any other please suggest
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.
09 August 2024 5,605 2 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?
06 August 2024 2,508 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds
04 August 2024 3,019 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Machine learning (ML) has shown great potential in predicting the compressive strength of concrete, an important property for structural engineering. However, its practical application comes with...
03 August 2024 2,546 2 View
I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....
31 July 2024 3,665 3 View
Hi All, Our Luminex 200 sat unused for a few years. After many sanitise/wash/alcohol flush cycles, and repeated stringent cleaning cycles, CAL2 always fails (The acquisition ran to sample empty)....
10 March 2024 4,055 0 View
Dear colleagues, We are planning to use Human XL Cytokine Base kit for evaluation of concentration of several cytokines. The plan was to measure concentration of cytokines both in blood serum, and...
20 February 2024 7,692 0 View
I have recently coupled some magplex beads to protein, using the biorad kit and beads following a standard protocol from my supervisor that has worked well previously. When I went to count these...
06 July 2023 1,794 1 View
I am losing beads in almost every well of 96 wells plate and also my beads per second is very low like highest was only 5. What could be the possible cause. I take appropriate time between the...
17 June 2023 1,168 2 View
I'm planning to process mouse inguinal and popliteal lymph nodes for Luminex assay. Does anyone have experience with it? What reagents to use? Right volume and dilution of the sample etc?
01 March 2023 4,663 0 View
I am looking for a commercially available GPL core antigen to use in a Luminex immunoassay. I am aware of the Capilia MAC Ab ELISA kit, but I am searching for just the antigen to couple to Luminex...
01 March 2023 9,433 0 View
Dear all, I intend to study T helper cells cytokines in mice pancreas tissue, please recommend a suitable buffer solution for homogenization step! In the protocol, they mentioned "For...
20 August 2022 5,065 0 View
What should be used as alternative cleaning fluid for luminex flow cytometer. I know that we can use contard 70, but not sure what percent, 10% or 15 % ? Do they have bleach in it with Luminex...
18 July 2022 1,427 1 View
We have compared a multiplexed luminex assay with the standard singleplex assays for ferritin, sTFR and RBP. The luminex values are manifold higher. We have used standards and purified proteins...
15 July 2022 5,265 0 View