I am looking for a commercially available GPL core antigen to use in a Luminex immunoassay. I am aware of the Capilia MAC Ab ELISA kit, but I am searching for just the antigen to couple to Luminex beads.
I apologize in advance for the length of this post but there is a lot of information. Our lab has an Acquity TQD coupled with an H-Class UPLC (Column manager-CM, sample manager-SM, and quaternary...
03 June 2024 6,448 3 View
If so do you have to crosslink the compound to the oligo? If you have done this kind of experiment I would really appreciate any guidance/protocols. Thanks, Katie
21 May 2024 318 1 View
i have recently done a disk diffusion assay where by i measure the zone of inhibition of 5 antibiotics on 7 different pathogens, i want to establish if there is any significant difference between...
12 March 2024 1,250 5 View
I am mixing a sodium alginate gel to apply to microfluidic channels, I’d like to use a fluorescent dye to visualise where it has been deposited after application. It would have to be oleophobic...
25 February 2024 8,205 1 View
I currently have some diffusion data for young children that we are trying to analyze. We are using FSL's eddy motion correction, but as we are looking at subcortical white matter pathways, we...
24 July 2023 1,951 2 View
I'm designing a new activity for an undergraduate biology course about enzyme activity. I'm running into a slight complication. I've ordered a lyophilized powdered version of human salivary...
12 July 2023 686 6 View
Has anyone analyzed intra- and inter-individual consistency in task order (i.e., repeatability)? I have repeated measures of multiple individuals and would like to see if a consistent action...
27 February 2023 337 3 View
I have asked my students to undertake differently worded questions on the same subject matter; Q1A & Q1B, Q2A & Q2B, Q3A &Q3B and so on, , I have then asked them to express agreement using a...
23 August 2022 7,724 8 View
Hi! I am measuring changes in glucose uptake in primary adult cardiomyocytes after addition of fatty acids. I am using a 1:100 dilution of CD lipids. Glucose uptake is measured by fluorescent...
14 July 2022 951 1 View
Hi! I'm isolating cardiomyocytes from adult mice and need to do live cell staining. I'd like to use myomesin as my cardiomyocyte marker (antibody: https://dshb.biology.uiowa.edu/mMaC-myomesin-B4)....
11 July 2022 6,005 0 View
I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....
31 July 2024 3,665 3 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
Hello! I planned to do an multiplex RNAscope fluorescent assay for cultured cells in a 96 well plate format according to the protocol provided by ACD:...
22 July 2024 3,334 0 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...
14 July 2024 6,724 0 View
Hello, I've been doing free floating IHC using a VAChT antibody with a 1:1k dilution (139 103 - Synaptic Systems). However, there has been a struggle to keep an even stain with my samples of...
06 July 2024 4,521 2 View
I would like to find a short course focused on the development of monoclonal antibodies covering the entire process, from the selection of antigen and carrier, to the purification of the final...
27 June 2024 5,618 1 View
I am trying to quantify the release of Nitric oxide to test the Immunogenicity of my antigen on U937 cells (Monocytes). Based on the literature, I am supposed to convert 1x105 cells/ml to...
27 June 2024 3,131 2 View
Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?
23 June 2024 2,264 4 View
If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...
17 June 2024 6,705 1 View