How to write multiple hypotheses?

This is what I have for my hypotheses at the moment - is this an okay to write it or how should I format it (this makes sense to me but I haven't seen hypotheses written like this before)? Any...

15 November 2020 994 2 View

Lipoic acid with a terminal amine causing gelation?

I am wondering if anyone has made Lipoic acid with a terminal amine and has seen gelation? Is there anyway around this or a way to get it back into solution?

29 October 2020 2,839 1 View

Should I include irrelevant parts of my methodology?

I am writing up a project in which I used motion capture as well as heart rate monitoring and questionnaires, but I will be analyzing the motion capture data separately to this project write up -...

23 October 2020 6,796 5 View

Is my research title too complex?

Research title: The effectiveness of gamified virtual reality for increasing adherence to exercise, affecting perceived exertion and improving mental and physical health: A preliminary study...

18 October 2020 4,505 10 View

Can you do multivariate multiple linear regression in AMOS?

Hello there, I have been struggling to wrap my head around which statistical analysis to use for my research question: I have 17-20 potential outcome variables (subtests on a cognitive battery,...

10 September 2019 9,953 3 View

Flood Prediction: Where should my starting point be?

Hi, I'm currently studying computer science and I am working on an Internet of Things project involving the use of rainfall sensors in different locations. I'm currently researching if these...

28 January 2019 864 5 View

Can you do immunofluorescence on slides that have already been stained by Diff-Quik?

Slides of BALF were stained with Diff-Quik. Can we stain the same slides for immunofluorescence?

05 September 2018 8,266 1 View

What are your suggestions to close up a vector after removing a fragment by restriction digest?

Dear community, I am working with a HUGE protien, in a plasmid we received from collaborators. It is 20,880 bp in size, and the gene of interest is aprox. 15,600 bp. Thankfully the received...

20 August 2018 9,664 4 View

When dissolving expanded polystyrene (EPS) in acetone what fumes are produced? And is there a way of measuring this?

I am researching and developing alternate methods of recycling EPS and am getting interesting results via dissolution in acetone. However I want to make an evaluation of the potentially hazardous...

15 January 2018 478 2 View

Can anyone give advice for doing mixed ANOVA with an imputed data set?

I have successfully imputed missing data in my data set but I am struggling to follow VanGinkel & Kroonenberg's instructions for what to do next. Does anyone know is there an easy way to do...

12 March 2017 4,817 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View