Does anyone have experience extracting proteins from mouse lymph nodes for Luminex assay?

02 March 2023 0 5K Report

I'm planning to process mouse inguinal and popliteal lymph nodes for Luminex assay. Does anyone have experience with it? What reagents to use? Right volume and dilution of the sample etc?

Badges
Science topic

Similar topics
Microbiology
Assays

More Mate Vadovics's questions See All

Is someone suggest how citations are increased?

how citations are increasing

24 April 2023 8,865 2 View

What is the best method to hexa meshing the lumbar spine?

I would like to mesh the human lumbar spine with hexahedral elements in HyperMesh/HyperWorks. Does anyone know a good article which describes the steps or even show it? Any help will be highly...

24 May 2021 7,004 0 View

How to assign the spring characteristic to the lumbar ligaments?

Hey! I would like to assign the nonlinear force-deflection connection to simulate the behaviour of the lumbar ligaments. I have the formula for the whole ligaemnt (e.g. Rohlmann et al. 2006). I...

27 February 2021 3,365 0 View

What is the best way to obtain merged images of the entire Hippocampus after Immunohistochemistry?

So, basically I would like to analyze the entire Hippocampus concerning the number of cells showing a certain stain. However, with the magnification I need to differentiate between the cells, the...

23 July 2020 560 2 View

How to take in a vacuum sublimator to an inert glovebox?

During my work, I have to do a reaction with the total exclusion of water. For this I use absolutized solvents and an inert Schleink technique. At the end of the reaction I should do a vacuum...

19 June 2020 8,631 0 View

How to estimate regression to find which firm-specific characteristics explain cross-industry heterogeneity in the firm returns?

I would kindly like to ask for guidance regarding my question below. To illustrate my question: I have two industries: Industry 1 and Industry 2. Both industries contain a number of firms that...

20 May 2019 5,120 1 View

Could someone suggest some papers about the maternal age-dependent aneuploidy rates in oocytes vs. embryos?

We would like to compare the aneuploidy rates of oocytes and embryos.

19 March 2019 4,020 2 View

In situ hybridization "resistant" genes in zebrafish - how to deal with them?

I would like to check the expression of two housekeeping genes, which, based on RT-PCR results, are expressed throughout development (most likely in a ubiquitous manner). Yet I just can't make the...

21 September 2016 9,462 5 View

What are existing approaches to design of behavioral interventions supported by web, mobile, and sensor technologies?

I am very much interested in the field of behavioral intervention research where much work has been done in order to decompose interventions into behavior change techniques in order to find out...

06 February 2016 6,369 5 View

What methods are used to establish the optimal sticker (vignette) prices for highway toll collection?

Vignette (sticker) is a form of road pricing imposed on vehicles, usually in addition to the compulsory road tax, based on a period of time instead of the usual road toll method based on distance...

25 September 2014 9,363 1 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended?

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...

05 August 2024 8,056 1 View

Can we use technical replicates for statistical analysis of multiplex cytokine data?

I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....

31 July 2024 3,665 3 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View