21 August 2022 0 5K Report

Dear all,

I intend to study T helper cells cytokines in mice pancreas tissue, please recommend a suitable buffer solution for homogenization step!

In the protocol, they mentioned "For cell/tissue homogenate, the final cell or tissue homogenate should be prepared in a buffer that has a neutral pH, contains minimal detergents or strong denaturing detergents, and has an ionic strength close to physiological concentration. Avoid debris, lipids, and cell/tissue aggregates. Centrifuge samples before use."

Thanks

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