I have recorded a presence of phytopathogenic microscopic fungi (including genera like Botrytis, Metasphaeria, Guignardia, Plasmopara or Sphaceloma) occured on Vitis vinifera and I want to isolate them in laboratory.
For the first step you can use potato dextrose agar (PDA) as a common medium for fungal isolation after sample preparation, sterilization and put on the medium in the petri dishes under laminair flow cabinet. In the final you should be incubate in the suitable temperature until your fungi grow..
In case Leptographium serpens (phytopathogenic fungi), the mycelium was obtained from logs of fire-injured P. pinaster trees. Tissues containing the fungus were placed onto 2% malt extract agar (MEA) and maintained at 25 ◦C in the dark for 2 weeks (Jacobs & Wingfield, 2001).