I have isolated many fungal isolates from diseased mangoes but when I am going to purified them these have been contaminated with mites. I don't know, How did these mites contaminated the cultures. How to get rid of this contamination.
Dear Uzma, when you left the plates of medium on the working table or else where un-protected, there will enter very small mites, that you may not see in routine. I recommend you to use parafilm wrapping on the plates which you put on working table for long period. I do recommend to wrap all the plates after streaking always.
IFTIKHAR
-------------------------------
IFTIKHAR AHMED, Ph.D.
Senior Scientific Officer
Post Doc Research Fellow
---------------------------
Office:
Room No. 221
Microbe Division / Japan Collection of Microorganism (JCM)
Riken BioResource Centre
3-1-1, Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
Phone: 0081-29-836-9549; Fax: 0081-29-836-9562
Mobile: 0081-80-4141-4950 (soft bank);
BrainTel: 0092-42-3 211 5039
Email: [email protected]
---------------------------
Affiliation:
Laboratory of Plant Nutrition and Fertilizers,
Department of Applied Biological Chemistry,
Graduate School of Agricultural and Life Sciences,
The University of Tokyo
Yayoi, Bunkyo-ku, Tokyo 113-8657, JAPAN
Tel +81-3-5841-5104 ; FAX +81-3-5841-8032
Email: [email protected]
---------------------------
Department Affiliation in Pakistan:
Plant Biotechnology Program,
National Institute for Genomics & Advanced Biotechnology (NIGAB),
National Agricultural Research Centre (NARC),
Park Road, Islamabad-45500
PAKISTAN
Phone: 0092-51-844 3706 (office)
0092-333-5134950 (Mobile)
Fax: 0092-51-9255 502
http://u-tokyo.academia.edu/IFTIKHARAHMED
http://www.researcherid.com/rid/B-4694-2009
Dear Sir, yes I do wrapped plates with parafilm and put them in incubator but still there is a problem of mite because these are produced whereever there is fungal growth even on fruits as well. So, I used camphor pellets placed near to fungal plates and this gave a very good result. But the camphor pelltes should be replaced by new one after a period of two to three weeks.
- Badges
- Science method