Is there anybody working in NIPT testing by NGS (Gene studio S5)?

we are planning to buy the gene studio S5 for starting the BIPT testing. we asked and inquired many times for knowing the cost per test for Nipt on above said instrument. i wish to know that what...

03 April 2019 581 0 View

Which machine is more better for real time PCR..?

i am going to buy a real time PCR machine for diagnostic purpose, so please suggest me which is advanced, better and more powerful for diagnostic purpose.

10 November 2018 8,100 5 View

Anybody can suggest a good Giemsa trypsin banding protocol ?

I am using SSC buffer to make a giemsa (20mg per 50 ml SSC buffer). we use aged slide (65 degree for overnight) for treating with Trypsin Solution for 20 sec at 20 degree. We get minor banding...

07 August 2018 4,400 4 View

PGMY11 primer set doesn't give any band with DNA sample which are shown positive with GP5+ and GP6+ primer set of cervical brush sample???

i screened many DNA samples extracted from the cervical brush sample for presence or absence of HPV DNA with primer set GP5+ /6+ and i got five positive sample. then i used that 5 positive samples...

07 August 2017 6,354 2 View

Any one have idea regarding sequence of Primers for Tuberculosis PCR?

There are many primers available in paper published, but most of all have problems with Tm, product size, nonspecific binding and many more. I need a best primer sequences for TB PCR. Is there...

02 March 2017 254 2 View

RAPD analysis of binomial matrix and dendogram preparation?

I have two variety, one is A - in vivo plant leaf and B - in vitro plant leaf. i had extracted DNA from these both samples A & B. Done PCR with 31 RAPD primers and got varies band pattern on...

08 September 2016 7,948 6 View

16s r-DNA PCR product giving three bands on gel, so which one should be considered?

I have Extracted DNA from six different actinomycetes bacterial strains and then do PCR with 27F & 1492R primers. i should get the band with size between 1400 to 1500 bp. but here i got three...

07 August 2016 9,941 6 View

RAPD analysis for the in-vitro and in-vivo plant samples of the same plant species?

I have done RAPD Analysis for one plant with its Invivo tissue and in vitro tissue samples. I have used 31 primer sets, so now i dont getting how much maximum variation can be considered for this...

07 August 2016 825 5 View

What should be the concentration and content of all PCR components for doing RAPD analysis?

i am going to do RAPD analysis of two different plant species with 31 different RAPD primers. I need to know the best suitable concentration and volume of primer, MgCl2, Taq pol, PCR buffer, and...

05 June 2016 914 3 View

How can I remove a high polysaccharide content from DNA extracted with plant leaf?

I have extracted plant DNA from leaf sample. i got good precipitates when precipitated with ethanol and iso propanol. but when i dissolve the dried pellet in TE or molecular grade water, it became...

11 December 2015 4,297 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View