I have extracted plant DNA from leaf sample. i got good precipitates when precipitated with ethanol and iso propanol. but when i dissolve the dried pellet in TE or molecular grade water, it became a very sticky and viscous. even that can not able to run in gel. when i performed gel electrophoresis, samples couldn't came out from well because of stickiness.
so is there any method or any protocol to remove poly saccharide content from extracted and dissolved DNA in TE / Water.
The last time I encountered this problem I think I had to use the bead based promega kit. I was using the columns but I seem to remember they clogged.
I think you should try the CTAB extraction as suggested, or you could also prepare your extraction reagent afresh:
Another option is to perform serial dilution with your extracted DNA before running it on the gel, to prevent the stickiness and also to enable the NA to migrate from the slot
You could try this protocol, which is very useful for plant total DNA extraction.
3% CTAB DNA extracion protocol
(modified from Zeng et al. 2002, ACta Botanica Sinica 44; 694-697)
1. washing out polysaccharides with CTAB-free buffer from homogenate.
2. increasing the percentage of CTAB in extraction medium.
3. using high concentration salts pior to DNA precipitation with isopanol.
- Badges
- Science method